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Literature summary for 3.2.1.1 extracted from

  • Mar, S.S.; Mori, H.; Lee, J.H.; Fukuda, K.; Saburi, W.; Fukuhara, A.; Okuyama, M.; Chiba, S.; Kimura, A.
    Purification, characterization, and sequence analysis of two alpha-amylase isoforms from azuki bean, Vigna angularis, showing different affinity towards beta-cyclodextrin sepharose (2003), Biosci. Biotechnol. Biochem., 67, 1080-1093.
    View publication on PubMed
Search for more literature for 3.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 inhibition above 100 mM, approx. 50% inhibition at 500 mM Vigna angularis
EDTA 10 mM, 5% inactivation after 5 min, complete inactivation after 4 h Vigna angularis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 amylose 0.1 mg/ml, 37°C, pH 5.3 Vigna angularis
additional information
-
 amylose 0.11 mg/ml, 37°C, pH 5.3 Vigna angularis
additional information
-
 p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 0.22 mg/ml, 37°C, pH 5.3 Vigna angularis
additional information
-
 p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 0.37 mg/ml, 37°C, pH 5.3 Vigna angularis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, SDS-PAGE Vigna angularis
47000
-
 x * 47000, SDS-PAGE Vigna angularis

Organism

Organism UniProt Comment Textmining
Vigna angularis
-
 azuki bean
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, beta-cyclodextrin Sepharose 6B Vigna angularis

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
63
-
-
 Vigna angularis
75
-
-
 Vigna angularis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,6-O-benzylidene 4-nitrophenyl-alpha-D-maltoheptaoside + H2O
-
 Vigna angularis 4,6-O-benzylidene 4-nitrophenyl-alpha-D-maltosides
-
 ?
amylose + H2O
-
 Vigna angularis fragments of amylose
-
 ?
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + H2O
-
 Vigna angularis p-nitrophenyl-alpha-D-glucopyranoside + D-glucose + ? predominant product, no p-nitrophenol detected ?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Vigna angularis
? x * 44000, SDS-PAGE Vigna angularis

Synonyms

Synonyms Comment Organism
VAAmy1
-
 Vigna angularis
VAAmy2
-
 Vigna angularis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Vigna angularis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 no loss of activity Vigna angularis
80
-
 complete loss of activity after 30 min Vigna angularis
80
-
 50% loss of activity after 30 min Vigna angularis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
55.5
-
 amylose 37°C, pH 5.3 Vigna angularis
62.4
-
 p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 37°C, pH 5.3 Vigna angularis
71.8
-
 amylose 37°C, pH 5.3 Vigna angularis
85.3
-
 p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 37°C, pH 5.3 Vigna angularis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Vigna angularis
5.3
-
-
 Vigna angularis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.9 11.9 20% loss of activity after 24 h at 4°C Vigna angularis
4.6 10.4 20% loss of activity after 24 h at 4°C Vigna angularis

pI Value

Organism Comment pI Value Maximum pI Value
Vigna angularis isoelectric focusing
-
 4.9