Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM CaCl2, inhibition of enzyme form Amyl II and Amyl III, no inhibition of enzyme form Amyl I | Aspergillus awamori | |
Cu2+ | 10 mM, strong inhibition of enzyme form Amyl I, Amyl II and Amyl III | Aspergillus awamori | |
Hg2+ | 10 mM, strong inhibition of enzyme form Amyl I, Amyl II and Amyl III | Aspergillus awamori | |
Zn2+ | 10 mM, strong inhibition of enzyme form Amyl I, Amyl II and Amyl III | Aspergillus awamori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Aspergillus awamori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activates | Aspergillus awamori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
enzyme form Amyl I, gel filtration | Aspergillus awamori |
59000 | - |
enzyme form Amyl II, gel filtration | Aspergillus awamori |
120000 | - |
enzyme form Amyl III, gel filtration | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Aspergillus awamori KT-11 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | enzyme form Amyl I contains 10.4% carbohydrate, enzyme form Amyl II contains 6.3% carbohydrate and enzyme form Amyl III 11.3% carbohydrate | Aspergillus awamori |
Purification (Comment) | Organism |
---|---|
three enzyme forms: Amyl I, Amyl II and Amyl III | Aspergillus awamori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclodextrin + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltoheptaose + H2O | - |
Aspergillus awamori | additional information | - |
? | |
maltohexaose + H2O | - |
Aspergillus awamori | additional information | - |
? | |
maltohexaose + H2O | - |
Aspergillus awamori KT-11 | additional information | - |
? | |
maltopentaose + H2O | - |
Aspergillus awamori | additional information | - |
? | |
maltopentaose + H2O | - |
Aspergillus awamori KT-11 | additional information | - |
? | |
maltotetraose + H2O | - |
Aspergillus awamori | additional information | - |
? | |
maltotetraose + H2O | - |
Aspergillus awamori KT-11 | additional information | - |
? | |
starch + H2O | soluble starch | Aspergillus awamori | additional information | enzyme form Amyl I hydrolyzes waxy maize starch to produce mainly maltose, maltotriose, and some maltooligosaccharides with higher degree of polymerization, the enzyme forms Amyl II and Amyl III produce maltose and some maltooligosaccharides of higher molecular weight than maltotetraose | ? | |
starch + H2O | waxy corn starch | Aspergillus awamori | additional information | enzyme form Amyl I hydrolyzes waxy maize starch to produce mainly maltose, maltotriose, and some maltooligosaccharides with higher degree of polymerization, the enzyme forms Amyl II and Amyl III produce maltose and some maltooligosaccharides of higher molecular weight than maltotetraose | ? | |
starch + H2O | soluble starch | Aspergillus awamori KT-11 | additional information | enzyme form Amyl I hydrolyzes waxy maize starch to produce mainly maltose, maltotriose, and some maltooligosaccharides with higher degree of polymerization, the enzyme forms Amyl II and Amyl III produce maltose and some maltooligosaccharides of higher molecular weight than maltotetraose | ? | |
starch + H2O | waxy corn starch | Aspergillus awamori KT-11 | additional information | enzyme form Amyl I hydrolyzes waxy maize starch to produce mainly maltose, maltotriose, and some maltooligosaccharides with higher degree of polymerization, the enzyme forms Amyl II and Amyl III produce maltose and some maltooligosaccharides of higher molecular weight than maltotetraose | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 59000, enzyme form Amyl II, SDS-PAGE | Aspergillus awamori |
monomer | 1 * 120000, enzyme form Amyl III, SDS-PAGE | Aspergillus awamori |
monomer | 1 * 46000, enzyme form Amyl I, SDS-PAGE | Aspergillus awamori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
enzyme form Amyl I | Aspergillus awamori |
60 | - |
enzyme form Amyl II and Amyl III | Aspergillus awamori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
enzyme form Amyl I | Aspergillus awamori |
5.5 | - |
enzyme form Amyl II and Amyl III | Aspergillus awamori |