Literature summary for 3.2.1.1 extracted from

Pytelkova, J.; Hubert, J.; Lepsik, M.; Sobotnik, J.; Sindelka, R.; Krizkova, I.; Horn, M.; Mares, M.
Digestive alpha-amylases of the flour moth Ephestia kuehniella--adaptation to alkaline environment and plant inhibitors (2009), FEBS J., 276, 3531-3546.

Search for more literature for 3.2.1.1

Inhibitors

Inhibitors	Comment	Organism	Structure
acarbose	-	Ephestia kuehniella
additional information	isozyme Amy3 is not inhibited by the proteinaceous inhibitor from bean alphaAI-1; isozymes Amy1 and Amy2 are not inhibited by the proteinaceous inhibitor from bean alphaAI-1 and by the proteinaceous inhibitor from wheat WI-3	Ephestia kuehniella
proteinaceous inhibitor from wheat WI-1	inhibitor of isozyme Amy2 but not Amy1	Ephestia kuehniella
proteinaceous inhibitor from wheat WI-3	-	Ephestia kuehniella

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	isozyme Amy3 is a chloride-independent alpha-amylase	Ephestia kuehniella

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54440	-	isozyme Amy3, calculated from amino acid sequence	Ephestia kuehniella
56000	-	isozyme Amy3, SDS-PAGE	Ephestia kuehniella

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Remazol Brilliant Blue dyed starch + H2O	Ephestia kuehniella	-	malto-oligosaccharides	-	?
Remazol Brilliant Blue-dyed starch + H2O	Ephestia kuehniella	-	malto-oligosaccharides	-	?

Organism

Organism	UniProt	Comment	Textmining
Ephestia kuehniella	-	flour moth	-
Ephestia kuehniella	B8Y698	isozyme Amy3; flour moth	-

Purification (Commentary)

Purification (Comment)	Organism
Mono Q column chromatography	Ephestia kuehniella

Source Tissue

Source Tissue	Comment	Organism	Textmining
larva	-	Ephestia kuehniella	-
midgut	-	Ephestia kuehniella	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Remazol Brilliant Blue dyed starch + H2O	-	Ephestia kuehniella	malto-oligosaccharides	-	?
Remazol Brilliant Blue-dyed starch + H2O	-	Ephestia kuehniella	malto-oligosaccharides	-	?

Synonyms

Synonyms	Comment	Organism
alpha-1,4-glucan-4-glucanohydrolase	-	Ephestia kuehniella
AMY1	isozyme	Ephestia kuehniella
AMY2	isozyme	Ephestia kuehniella
Amy3	isozyme	Ephestia kuehniella

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	isozyme Amy3	Ephestia kuehniella
9.5	-	isozymes Amy1 and Amy2	Ephestia kuehniella

pH Range

pH Minimum	pH Maximum	Comment	Organism
8	11	-	Ephestia kuehniella

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.000008	-	isozyme Amy3, in 0.1 M Britton-Robinson buffer at pH 8.0, at 26°C	Ephestia kuehniella	proteinaceous inhibitor from wheat WI-1
0.00103	-	isozyme Amy3, in 0.1 M Britton-Robinson buffer at pH 8.0, at 26°C	Ephestia kuehniella	proteinaceous inhibitor from wheat WI-3
0.0021	-	isozyme Amy3, in 0.1 M Britton-Robinson buffer at pH 8.0, at 26°C	Ephestia kuehniella	acarbose
0.0056	-	isozyme Amy2, in 0.1 M Britton-Robinson buffer at pH 9.5, at 26°C	Ephestia kuehniella	acarbose
0.00579	-	isozyme Amy2, in 0.1 M Britton-Robinson buffer at pH 9.5, at 26°C	Ephestia kuehniella	proteinaceous inhibitor from wheat WI-1
0.01362	-	isozyme Amy1, in 0.1 M Britton-Robinson buffer at pH 9.5, at 26°C	Ephestia kuehniella	acarbose

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Release 2023.1 (January 2023)