Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.1 extracted from

  • Ueda, M.; Asano, T.; Nakazawa, M.; Miyatake, K.; Inouye, K.
    Purification and characterization of novel raw-starch-digesting and cold-adapted alpha-amylases from Eisenia foetida (2008), Comp. Biochem. Physiol. B, 150, 125-130.
    View publication on PubMed

Application

Application Comment Organism
industry could be useful in industrial operations such as brewing and food processing, due to their activity at low and high temperatures Eisenia fetida

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, pH 8.0, 24 h at 4°C, 110% and 73% residual activity for Amy I and Amy II, respectively Eisenia fetida
Ba2+ 1 mM, pH 8.0, 24 h at 4°C, 104% and 95% residual activity for Amy I and Amy II, respectively Eisenia fetida
Co2+ 1 mM, pH 8.0, 24 h at 4°C, 92% and 93% residual activity for Amy I and Amy II, respectively Eisenia fetida
Cu2+ 1 mM, pH 8.0, 24 h at 4°C, 36% and 64% residual activity for Amy I and Amy II, respectively Eisenia fetida
EDTA 1 mM, pH 8.0, 24 h at 4°C, 98% and 76% residual activity for Amy I and Amy II, respectively Eisenia fetida
Fe2+ 1 mM, pH 8.0, 24 h at 4°C, 68% and 24% residual activity for Amy I and Amy II, respectively Eisenia fetida
Hg2+ 1 mM, pH 8.0, 24 h at 4°C, 35% and 31% residual activity for Amy I and Amy II, respectively Eisenia fetida
Pb2+ 1 mM, pH 8.0, 24 h at 4°C, 101% and 87% residual activity for Amy I and Amy II, respectively Eisenia fetida
Zn2+ 1 mM, pH 8.0, 24 h at 4°C, 94% and 64% residual activity for Amy I and Amy II, respectively Eisenia fetida

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ 1 mM, pH 8.0, 24 h at 4°C, slight activation for Amy I and Amy II Eisenia fetida
Al3+ 1 mM, pH 8.0, 24 h at 4°C, 110% and 73% residual activity for Amy I and Amy II, respectively Eisenia fetida
Ba2+ 1 mM, pH 8.0, 24 h at 4°C, 104% and 95% residual activity for Amy I and Amy II, respectively Eisenia fetida
Ca2+ 1 mM, pH 8.0, 24 h at 4°C, 105% and 106% residual activity for Amy I and Amy II, respectively Eisenia fetida
Mg2+ 1 mM, pH 8.0, 24 h at 4°C, 111% and 107% residual activity for Amy I and Amy II, respectively Eisenia fetida
Mn2+ 1 mM, pH 8.0, 24 h at 4°C, 108% and 102% residual activity for Amy I and Amy II, respectively Eisenia fetida
Pb2+ 1 mM, pH 8.0, 24 h at 4°C, 101% and 87% residual activity for Amy I and Amy II, respectively Eisenia fetida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
SDS-PAGE Eisenia fetida

Organism

Organism UniProt Comment Textmining
Eisenia fetida
-
earthworm
-

Purification (Commentary)

Purification (Comment) Organism
Amy I, 85.2fold purified and Amy II, 263fold purified Eisenia fetida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
18.7 U/mg, purified Amy II Eisenia fetida
additional information
-
6.05 U/mg, purifed Amy II Eisenia fetida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity detected using cassava starch as substrate for Amy I Eisenia fetida ?
-
?
starch + H2O various sources of starch are tested, japonica rice as control with 100% relative activity, India rice: 294% and 508% relative activity for Amy I and Amy II, respectively, corn: 10.7% and 2.8% relative activity for Amy I and Amy II, respectively, wheat: 27.1% and 47.5% relative activity for Amy I and Amy II, respectively, potato: 10.6% and 4.47% relative activity for Amy I and Amy II, respectively, sweet potato: 13.5% and 1.88% relative activity for Amy I and Amy II, respectively, cassava: 1.22% relative activity for Amy II Eisenia fetida D-glucose + maltose + maltotriose reactions product identified by thin-layer-chromatography ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Eisenia fetida

Synonyms

Synonyms Comment Organism
Amy I
-
Eisenia fetida
Amy II
-
Eisenia fetida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Eisenia fetida
50
-
Amy I and Amy II Eisenia fetida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 Amy I and Amy II, 20°C: ca. 40% and ca. 50% relative activity of Amy I and Amy II, respectively, 70°C: ca. 60% and ca. 40% relative activity of Amy I and Amy II, respectively Eisenia fetida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 60 Amy I and Amy II, pH 8.0, 30 min, 10°C: ca. 90% relative activity for both Amy I and Amy II, 60°C: ca. 10% and ca. 90% relative activity for Amy I and Amy II, respectively, maximal stability between 50 and 60°C Eisenia fetida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at, optimum for Amy I and Amy II, showing at least 95% of their maximum activities over a pH range from pH 7.0 to 9.0 Eisenia fetida

pH Range

pH Minimum pH Maximum Comment Organism
4 9 Amy I, pH 4: ca. 30% relative activity, pH 9: ca. 40% relative activity Eisenia fetida
5 7 Amy II, pH 5.0: ca. 50% relative activity, pH 7.0: ca. 40% relative activity Eisenia fetida

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 Amy II, 24 h at 4°C, pH 5, ca. 42% residual activity, pH 9: ca. 75% residual activity, maximal stability between pH 7 and 9 Eisenia fetida
5 10 Amy I, 24 h at 4°C, pH 5, ca. 22% residual activity, pH 10: ca. 10% residual activity, maximal stability between pH 7 and 9 Eisenia fetida