Application | Comment | Organism |
---|---|---|
industry | could be useful in industrial operations such as brewing and food processing, due to their activity at low and high temperatures | Eisenia fetida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 1 mM, pH 8.0, 24 h at 4°C, 110% and 73% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Ba2+ | 1 mM, pH 8.0, 24 h at 4°C, 104% and 95% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Co2+ | 1 mM, pH 8.0, 24 h at 4°C, 92% and 93% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Cu2+ | 1 mM, pH 8.0, 24 h at 4°C, 36% and 64% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
EDTA | 1 mM, pH 8.0, 24 h at 4°C, 98% and 76% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Fe2+ | 1 mM, pH 8.0, 24 h at 4°C, 68% and 24% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Hg2+ | 1 mM, pH 8.0, 24 h at 4°C, 35% and 31% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Pb2+ | 1 mM, pH 8.0, 24 h at 4°C, 101% and 87% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Zn2+ | 1 mM, pH 8.0, 24 h at 4°C, 94% and 64% residual activity for Amy I and Amy II, respectively | Eisenia fetida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, pH 8.0, 24 h at 4°C, slight activation for Amy I and Amy II | Eisenia fetida | |
Al3+ | 1 mM, pH 8.0, 24 h at 4°C, 110% and 73% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Ba2+ | 1 mM, pH 8.0, 24 h at 4°C, 104% and 95% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Ca2+ | 1 mM, pH 8.0, 24 h at 4°C, 105% and 106% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Mg2+ | 1 mM, pH 8.0, 24 h at 4°C, 111% and 107% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Mn2+ | 1 mM, pH 8.0, 24 h at 4°C, 108% and 102% residual activity for Amy I and Amy II, respectively | Eisenia fetida | |
Pb2+ | 1 mM, pH 8.0, 24 h at 4°C, 101% and 87% residual activity for Amy I and Amy II, respectively | Eisenia fetida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Eisenia fetida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eisenia fetida | - |
earthworm | - |
Purification (Comment) | Organism |
---|---|
Amy I, 85.2fold purified and Amy II, 263fold purified | Eisenia fetida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
18.7 U/mg, purified Amy II | Eisenia fetida |
additional information | - |
6.05 U/mg, purifed Amy II | Eisenia fetida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity detected using cassava starch as substrate for Amy I | Eisenia fetida | ? | - |
? | |
starch + H2O | various sources of starch are tested, japonica rice as control with 100% relative activity, India rice: 294% and 508% relative activity for Amy I and Amy II, respectively, corn: 10.7% and 2.8% relative activity for Amy I and Amy II, respectively, wheat: 27.1% and 47.5% relative activity for Amy I and Amy II, respectively, potato: 10.6% and 4.47% relative activity for Amy I and Amy II, respectively, sweet potato: 13.5% and 1.88% relative activity for Amy I and Amy II, respectively, cassava: 1.22% relative activity for Amy II | Eisenia fetida | D-glucose + maltose + maltotriose | reactions product identified by thin-layer-chromatography | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Eisenia fetida |
Synonyms | Comment | Organism |
---|---|---|
Amy I | - |
Eisenia fetida |
Amy II | - |
Eisenia fetida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Eisenia fetida |
50 | - |
Amy I and Amy II | Eisenia fetida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | Amy I and Amy II, 20°C: ca. 40% and ca. 50% relative activity of Amy I and Amy II, respectively, 70°C: ca. 60% and ca. 40% relative activity of Amy I and Amy II, respectively | Eisenia fetida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | Amy I and Amy II, pH 8.0, 30 min, 10°C: ca. 90% relative activity for both Amy I and Amy II, 60°C: ca. 10% and ca. 90% relative activity for Amy I and Amy II, respectively, maximal stability between 50 and 60°C | Eisenia fetida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at, optimum for Amy I and Amy II, showing at least 95% of their maximum activities over a pH range from pH 7.0 to 9.0 | Eisenia fetida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | Amy I, pH 4: ca. 30% relative activity, pH 9: ca. 40% relative activity | Eisenia fetida |
5 | 7 | Amy II, pH 5.0: ca. 50% relative activity, pH 7.0: ca. 40% relative activity | Eisenia fetida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | Amy II, 24 h at 4°C, pH 5, ca. 42% residual activity, pH 9: ca. 75% residual activity, maximal stability between pH 7 and 9 | Eisenia fetida |
5 | 10 | Amy I, 24 h at 4°C, pH 5, ca. 22% residual activity, pH 10: ca. 10% residual activity, maximal stability between pH 7 and 9 | Eisenia fetida |