Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
A53S | in comparison to the wild type, calcium ion has more effect on the catalytic efficiency, kcat/Km, and half-life (at 60°C) of A53S mutant, although the overall activity (kcat/Km) has not improved, about 80% of activity is maintained in the case of A53S mutant | Priestia megaterium |
H58I | calcium-independent mutant possessing high thermostability, in the absence of CaCl2, the H58I mutant is much more stable than the wild type and there is a 7fold increase in the residual activity of H58I mutant after 30 min of incubation as compared to the wild type | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | in the presence of calcium, the affinity of the enzymes (wild type and mutants) toward starch is increased, the thermostability of the wild type and A53S mutant is calcium dependent at different temperatures, in the presence of 2 mM CaCl2 at 60°C, the percentage of residual activity in both the wild type and A53S mutant changes after 30 min of incubation | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
predicted from amino acid sequence, SDS-PAGE | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | P20845 | - |
- |
Priestia megaterium WHO | P20845 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, gel filtration | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Priestia megaterium | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Priestia megaterium WHO | malto-oligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,4-alpha-D-glucan glucanohydrolase | - |
Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
in the absence of Ca2+ | Priestia megaterium |
60.7 | - |
in the presence of 5 mM Ca2+ | Priestia megaterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.8 | - |
starch | mutant enzyme A53S, in the presence of 5 mM Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C | Priestia megaterium | |
19.9 | - |
starch | wild type enzyme, in the presence of 5 mM Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C | Priestia megaterium | |
20.7 | - |
starch | mutant enzyme H58I, in the presence of 5 mM Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C | Priestia megaterium | |
22.4 | - |
starch | mutant enzyme A53S, in the absence of Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C | Priestia megaterium | |
25.7 | - |
starch | wild type enzyme, in the absence of Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C | Priestia megaterium | |
27.4 | - |
starch | mutant enzyme H58I, in the absence of Ca2+, in 20 mM Tris-HCl buffer (pH 7.2), at 30°C | Priestia megaterium |