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Literature summary for 3.2.1.1 extracted from

  • Yang, C.H.; Huang, Y.C.; Chen, C.Y.; Wen, C.Y.
    Heterologous expression of Thermobifida fusca thermostable alpha-amylase in Yarrowia lipolytica and its application in boiling stable resistant sago starch preparation (2010), J. Ind. Microbiol. Biotechnol., 37, 953-960.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
high level recombinant expression in Yarrowia lipolytica strain P01g using the vector pYLSC1 allowing constitutive expression and secretion of the protein Thermobifida fusca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, recombinant enzyme, SDS-PAGE Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca Q1KLC8 isolated from compost soils collected in Taiwan
-
Thermobifida fusca NTU22 Q1KLC8 isolated from compost soils collected in Taiwan
-

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular enzyme expressed from Yarrowia lipolytica strain P01g 10.8fold by ultrafiltration, anion exchange chromatography, and gel filtration Thermobifida fusca

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
61.65
-
purified recombinant enzyme, pH 7.0, 60°C Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
raw sago starch + H2O high level of activity Thermobifida fusca ?
-
?
raw sago starch + H2O high level of activity Thermobifida fusca NTU22 ?
-
?

Subunits

Subunits Comment Organism
? x * 65000, recombinant enzyme, SDS-PAGE Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified recombinant enzyme, 3 h, 70% activity remaining Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Thermobifida fusca