Cloned (Comment) | Organism |
---|---|
high level recombinant expression in Yarrowia lipolytica strain P01g using the vector pYLSC1 allowing constitutive expression and secretion of the protein | Thermobifida fusca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, recombinant enzyme, SDS-PAGE | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | Q1KLC8 | isolated from compost soils collected in Taiwan | - |
Thermobifida fusca NTU22 | Q1KLC8 | isolated from compost soils collected in Taiwan | - |
Purification (Comment) | Organism |
---|---|
recombinant extracellular enzyme expressed from Yarrowia lipolytica strain P01g 10.8fold by ultrafiltration, anion exchange chromatography, and gel filtration | Thermobifida fusca |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61.65 | - |
purified recombinant enzyme, pH 7.0, 60°C | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
raw sago starch + H2O | high level of activity | Thermobifida fusca | ? | - |
? | |
raw sago starch + H2O | high level of activity | Thermobifida fusca NTU22 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, recombinant enzyme, SDS-PAGE | Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant enzyme, 3 h, 70% activity remaining | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermobifida fusca |