Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.1 extracted from

  • Uma Maheswar Rao, J.L.; Satyanarayana, T.
    Purification and characterization of a hyperthermostable and high maltogenic alpha-amylase of an extreme thermophile Geobacillus thermoleovorans (2007), Appl. Biochem. Biotechnol., 142, 179-193.
    View publication on PubMed

General Stability

General Stability Organism
polyethylene glycol 8000 and Tween 20, 40, and 80 stabilized the enzyme activity Geobacillus thermoleovorans

Inhibitors

Inhibitors Comment Organism Structure
Benzene 10%, 40% inhibition Geobacillus thermoleovorans
butanol 10%, 80% inhibition Geobacillus thermoleovorans
Dextran 1%, 20% inhibition Geobacillus thermoleovorans
dithiothreitol 1 mM, 50% inhibition Geobacillus thermoleovorans
EDTA 1 mM, 36% inhibition Geobacillus thermoleovorans
ethanol 10%, 36% inhibition Geobacillus thermoleovorans
glycerol 1%, 26% inhibition Geobacillus thermoleovorans
glycine 2%, 20% inhibition Geobacillus thermoleovorans
Hg2+ 1 mM, complete inhibition Geobacillus thermoleovorans
iodoacetate 1 mM, 60% inhibition Geobacillus thermoleovorans
methanol 20%, 45% inhibition Geobacillus thermoleovorans
N-ethylmaleimide 1 mM, complete inhibition Geobacillus thermoleovorans
Pb2+ 5 mM, 80% inhibition Geobacillus thermoleovorans
phenyl methyl sulfonyl fluoride 1 mM, 70% inhibition Geobacillus thermoleovorans
Sn2+ 1 mM, complete inhibition Geobacillus thermoleovorans
Sodium dodecyl sulfate 0.2%, 58% inhibition Geobacillus thermoleovorans
Triton X-100 0.2%, 40% inhibition Geobacillus thermoleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information KM for soluble starch is 1.1 mg/ml, KM-value for potato starch or ice starch is 0.9 mg/ml Geobacillus thermoleovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 1 mM, 2.24fold activation Geobacillus thermoleovorans
Co2+ 1 mM, 3.06fold activation Geobacillus thermoleovorans
Fe2+ 1 mM, 2.72fold activation Geobacillus thermoleovorans
Mg2+ 1 mM, 1.72fold activation Geobacillus thermoleovorans
Mn2+ 1 mM, 1.34fold activation Geobacillus thermoleovorans
Ni2+ 1 mM, 1.23fold activation Geobacillus thermoleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
gel filtration Geobacillus thermoleovorans

Organism

Organism UniProt Comment Textmining
Geobacillus thermoleovorans
-
-
-
Geobacillus thermoleovorans NP54
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus thermoleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
450 U/mg. One saccharogenic alpha-amylase unit is defined as the amount of enzyme required for the liberation of 0.001 mM of reducing sugars as maltose per milliliter per minute Geobacillus thermoleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-cyclodextrin + H2O 9.2% of the activity with soluble starch Geobacillus thermoleovorans ?
-
?
alpha-cyclodextrin + H2O 9.2% of the activity with soluble starch Geobacillus thermoleovorans NP54 ?
-
?
amylopectin + H2O 66.1% of the activity with soluble starch Geobacillus thermoleovorans ?
-
?
amylopectin + H2O 66.1% of the activity with soluble starch Geobacillus thermoleovorans NP54 ?
-
?
pullulan + H2O 41.1% of the activity with soluble starch Geobacillus thermoleovorans ?
-
?
pullulan + H2O 41.1% of the activity with soluble starch Geobacillus thermoleovorans NP54 ?
-
?
soluble starch + H2O
-
Geobacillus thermoleovorans ?
-
?
soluble starch + H2O
-
Geobacillus thermoleovorans NP54 ?
-
?
starch + H2O rice starch, starch of pearl millet, corn starch, potato starch, wheat starch Geobacillus thermoleovorans ?
-
?
starch + H2O rice starch, starch of pearl millet, corn starch, potato starch, wheat starch Geobacillus thermoleovorans NP54 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
Geobacillus thermoleovorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 110 70°C: aboutb 50% of maximal activity, 110°C: about 90% of maximal activity Geobacillus thermoleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
t1/2 increases from 3.6 h to 5.6 h in presence of cholic acid Geobacillus thermoleovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
98.3
-
soluble starch
-
Geobacillus thermoleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Geobacillus thermoleovorans

pI Value

Organism Comment pI Value Maximum pI Value
Geobacillus thermoleovorans isoelectric focusing, pH-gradient: 3-10
-
5.4