General Stability | Organism |
---|---|
polyethylene glycol 8000 and Tween 20, 40, and 80 stabilized the enzyme activity | Geobacillus thermoleovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Benzene | 10%, 40% inhibition | Geobacillus thermoleovorans | |
butanol | 10%, 80% inhibition | Geobacillus thermoleovorans | |
Dextran | 1%, 20% inhibition | Geobacillus thermoleovorans | |
dithiothreitol | 1 mM, 50% inhibition | Geobacillus thermoleovorans | |
EDTA | 1 mM, 36% inhibition | Geobacillus thermoleovorans | |
ethanol | 10%, 36% inhibition | Geobacillus thermoleovorans | |
glycerol | 1%, 26% inhibition | Geobacillus thermoleovorans | |
glycine | 2%, 20% inhibition | Geobacillus thermoleovorans | |
Hg2+ | 1 mM, complete inhibition | Geobacillus thermoleovorans | |
iodoacetate | 1 mM, 60% inhibition | Geobacillus thermoleovorans | |
methanol | 20%, 45% inhibition | Geobacillus thermoleovorans | |
N-ethylmaleimide | 1 mM, complete inhibition | Geobacillus thermoleovorans | |
Pb2+ | 5 mM, 80% inhibition | Geobacillus thermoleovorans | |
phenyl methyl sulfonyl fluoride | 1 mM, 70% inhibition | Geobacillus thermoleovorans | |
Sn2+ | 1 mM, complete inhibition | Geobacillus thermoleovorans | |
Sodium dodecyl sulfate | 0.2%, 58% inhibition | Geobacillus thermoleovorans | |
Triton X-100 | 0.2%, 40% inhibition | Geobacillus thermoleovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM for soluble starch is 1.1 mg/ml, KM-value for potato starch or ice starch is 0.9 mg/ml | Geobacillus thermoleovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, 2.24fold activation | Geobacillus thermoleovorans | |
Co2+ | 1 mM, 3.06fold activation | Geobacillus thermoleovorans | |
Fe2+ | 1 mM, 2.72fold activation | Geobacillus thermoleovorans | |
Mg2+ | 1 mM, 1.72fold activation | Geobacillus thermoleovorans | |
Mn2+ | 1 mM, 1.34fold activation | Geobacillus thermoleovorans | |
Ni2+ | 1 mM, 1.23fold activation | Geobacillus thermoleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
gel filtration | Geobacillus thermoleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermoleovorans | - |
- |
- |
Geobacillus thermoleovorans NP54 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus thermoleovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
450 U/mg. One saccharogenic alpha-amylase unit is defined as the amount of enzyme required for the liberation of 0.001 mM of reducing sugars as maltose per milliliter per minute | Geobacillus thermoleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-cyclodextrin + H2O | 9.2% of the activity with soluble starch | Geobacillus thermoleovorans | ? | - |
? | |
alpha-cyclodextrin + H2O | 9.2% of the activity with soluble starch | Geobacillus thermoleovorans NP54 | ? | - |
? | |
amylopectin + H2O | 66.1% of the activity with soluble starch | Geobacillus thermoleovorans | ? | - |
? | |
amylopectin + H2O | 66.1% of the activity with soluble starch | Geobacillus thermoleovorans NP54 | ? | - |
? | |
pullulan + H2O | 41.1% of the activity with soluble starch | Geobacillus thermoleovorans | ? | - |
? | |
pullulan + H2O | 41.1% of the activity with soluble starch | Geobacillus thermoleovorans NP54 | ? | - |
? | |
soluble starch + H2O | - |
Geobacillus thermoleovorans | ? | - |
? | |
soluble starch + H2O | - |
Geobacillus thermoleovorans NP54 | ? | - |
? | |
starch + H2O | rice starch, starch of pearl millet, corn starch, potato starch, wheat starch | Geobacillus thermoleovorans | ? | - |
? | |
starch + H2O | rice starch, starch of pearl millet, corn starch, potato starch, wheat starch | Geobacillus thermoleovorans NP54 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Geobacillus thermoleovorans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 110 | 70°C: aboutb 50% of maximal activity, 110°C: about 90% of maximal activity | Geobacillus thermoleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
t1/2 increases from 3.6 h to 5.6 h in presence of cholic acid | Geobacillus thermoleovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
98.3 | - |
soluble starch | - |
Geobacillus thermoleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Geobacillus thermoleovorans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus thermoleovorans | isoelectric focusing, pH-gradient: 3-10 | - |
5.4 |