Information on EC 3.2.1.39 - glucan endo-1,3-beta-D-glucosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.2.1.39
-
RECOMMENDED NAME
GeneOntology No.
glucan endo-1,3-beta-D-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
transglycosylation
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
3-beta-D-glucan glucanohydrolase
Different from EC 3.2.1.6 endo-1,3(4)-beta-glucanase. Very limited action on mixed-link (1->3,1->4)-beta-D-glucans. Hydrolyses laminarin, paramylon and pachyman.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-37-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
enzyme synthesis induced by culturing on media containing Agaricus bisporus fruiting body cell walls and fructose
-
-
Manually annotated by BRENDA team
strain ATCC 76739
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain NHB-10
Uniprot
Manually annotated by BRENDA team
member of glycosyl hydrolase family 81
-
-
Manually annotated by BRENDA team
enhanced activity if grown in the presence of NaCl
-
-
Manually annotated by BRENDA team
endophytic in Hevea brasiliensis
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
NM-1
-
-
Manually annotated by BRENDA team
NM-1
-
-
Manually annotated by BRENDA team
enzyme expression is decreased during the hyphal transition
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
enzyme comprises a catalytic domain of the glycoside hydrolase family 16 and a C-terminal carbohydrate-binding module family 13
-
-
Manually annotated by BRENDA team
mexican lime
-
-
Manually annotated by BRENDA team
gene CaLam
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Drosera rotundifolia Viku­lan Järvi
-
-
-
Manually annotated by BRENDA team
Eulota maakii
-
-
-
Manually annotated by BRENDA team
Flavobacterium dormitator
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Fungi imperfecti
non-identified strain
-
-
Manually annotated by BRENDA team
strain Pocock 1888
-
-
Manually annotated by BRENDA team
Gecarcoidea natalis Pocock 1888
strain Pocock 1888
-
-
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
Pacific abalone
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Glu1 does not belong to existing GH families containing EC 3.2.1.39 glucanases
UniProt
Manually annotated by BRENDA team
Littorina kurila
-
-
-
Manually annotated by BRENDA team
cv. Jonagold
-
-
Manually annotated by BRENDA team
AAA group, subgroup Cavendish, cv. Giant governor
-
-
Manually annotated by BRENDA team
different cultivars Rasthali, Kanthali, and Monthan
-
-
Manually annotated by BRENDA team
strain F96
-
-
Manually annotated by BRENDA team
strain F96
-
-
Manually annotated by BRENDA team
strain BCRC 17245, gene lamA
UniProt
Manually annotated by BRENDA team
member of glycosyl hydrolase family 16
-
-
Manually annotated by BRENDA team
isolated from female Long Evans rats, gene PCEng2
UniProt
Manually annotated by BRENDA team
cv. Bruine biggareau
-
-
Manually annotated by BRENDA team
gene RmLam81A
UniProt
Manually annotated by BRENDA team
gene RmLam81A
UniProt
Manually annotated by BRENDA team
Rhizopus sp.
-
-
-
Manually annotated by BRENDA team
strain F7, induction of enzyme by glucans containing 1-3-linkages
SwissProt
Manually annotated by BRENDA team
strain YPA84
-
-
Manually annotated by BRENDA team
defective in exo-beta-1,3-glucanase formation
-
-
Manually annotated by BRENDA team
Sclerotium glucanicum
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
(Moench) Garcke, strain Oberna behen (L.) Ikonn
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-
Manually annotated by BRENDA team
gene gluB20-2
UniProt
Manually annotated by BRENDA team
member of glycosyl hysrolase family 16
UniProt
Manually annotated by BRENDA team
grown on purified cell wall of Rhizotonia solani
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-
Manually annotated by BRENDA team
curdlan-hydrolyzing strain Mo is separated from soil
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-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
strain YX-ER1
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-
Manually annotated by BRENDA team
strain YX-ER1
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-
Manually annotated by BRENDA team
gene TmLam
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-
Manually annotated by BRENDA team
member of glycosyl hydrolase family 16
UniProt
Manually annotated by BRENDA team
member of glycosyl hydrolase family 16
UniProt
Manually annotated by BRENDA team
Trichoderma harzianum Rifai T24
Rifai T24
-
-
Manually annotated by BRENDA team
strain ALL 13
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-
Manually annotated by BRENDA team
strain ALL 13
-
-
Manually annotated by BRENDA team
isolated from Brazilian Cerrado soil
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
gene Zobellia_2431
UniProt
Manually annotated by BRENDA team
gene Zobellia_2431
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1-3)-beta-D-glucan + H2O
laminaritriose + laminaritetraose
show the reaction diagram
(1-3:1-4)-beta-D-glucan + H2O
?
show the reaction diagram
3-O-beta-D-Glc-D-Glc-D-Glc + H2O
?
show the reaction diagram
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O
?
show the reaction diagram
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc + H2O
?
show the reaction diagram
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc + H2O
?
show the reaction diagram
3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc-D-Glc + H2O
?
show the reaction diagram
-
laminariheptaose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
show the reaction diagram
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
show the reaction diagram
-
gentiobiose, low activity
-
?
alkali soluble 1,3-beta-glucan + H2O
reducing sugar + ?
show the reaction diagram
-
produced by solubilised 1,3-beta-glucan synthase complex of A. fumigatus
-
?
alpha-laminaribiosyl fluoride + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
azurine-crosslinked beta-1,3-glucan + H2O
?
show the reaction diagram
-
AZCL-pachyman
-
?
azurine-crosslinked beta-1,3:1,4-glucan + H2O
?
show the reaction diagram
-
AZCL-barley-beta-glucan, 100fold less active than on AZCL-pachyman
-
?
barley beta-glucan + H2O
?
show the reaction diagram
barley glucan + H2O
?
show the reaction diagram
-
8% activity compared to laminarin
-
-
?
beta-1,3-1,4-glucan + H2O
?
show the reaction diagram
substrate from Hordeum vulgare, substrate of EC 3.2.1.6, endo-1,3(4)-beta-glucanase
-
-
?
beta-1,3-glucan + H2O
?
show the reaction diagram
beta-1,3-glucan + H2O
laminaribiose + D-glucose
show the reaction diagram
beta-D-glucan + H2O
?
show the reaction diagram
beta-glucan + H2O
?
show the reaction diagram
callose + H2O
?
show the reaction diagram
-
-
-
?
carboxy-methylated pachyman + H2O
?
show the reaction diagram
carboxymethyl curdlan + H2O
?
show the reaction diagram
13.7% of the activity with laminarin
-
-
?
carboxymethyl curdlan-Remazol Brilliant Blue + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl pachyman + H2O
?
show the reaction diagram
carboxymethyl-cellulose + H2O
?
show the reaction diagram
-
carboxymethyl-cellulose is only hydrolyzed by YlCrh1Sp
-
-
?
carboxymethyl-curdlan + H2O
?
show the reaction diagram
-
-
-
?
carboxymethylated curdlan + H2O
?
show the reaction diagram
-
-
-
?
carboxymethylated pachyman + H2O
?
show the reaction diagram
-
-
-
?
carboxymethylated pachyman + H2O
D-glucose + ?
show the reaction diagram
carboxymethylated pachyman + H2O
oligoglucosides
show the reaction diagram
-
86% of activity compared to laminarin from Laminaria digitata
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
cell wall glucan + H2O
reducing sugar + ?
show the reaction diagram
chitin + H2O
?
show the reaction diagram
curdlan + H2O
?
show the reaction diagram
curdlan + H2O
D-glucose + laminaritriose + laminaribiose
show the reaction diagram
-
-
-
-
?
curdlan + H2O
laminarioligosaccharides + laminaribiose
show the reaction diagram
Glc-beta-(1->4)-Glc-beta-(1->3)-Glc-beta-(1->4)-Glc + H2O
D-glucose + Glc-beta-(1->4)-Glc-beta-(1->3)-Glc
show the reaction diagram
insoluble yeast glucan + H2O
?
show the reaction diagram
-
-
-
-
?
laminaran + H2O
?
show the reaction diagram
laminaran from Laminaria cichorioides + H2O
D-glucose + ?
show the reaction diagram
Laminaria digitata laminarin + H2O
laminarioligosaccharides
show the reaction diagram
laminariheptaose + H2O
?
show the reaction diagram
laminariheptaose + H2O
laminaripentaose + laminaribiose
show the reaction diagram
laminarihexaose + H2O
?
show the reaction diagram
laminarihexaose + H2O
laminaritetraose + laminaribiose
show the reaction diagram
laminarihexose + H2O
laminaritriose + laminaritetraose
show the reaction diagram
-
active with the wild-type enzyme, but also the active site mutant E259A, that shows residual activity in the substrate complex crystal and cleaves the substrate in two different ways, generating trisaccharides and tetrasaccharides, mass spectrometry
laminaritriose shows higher binding affinity and fully occupies the -1, -2 and -3 sites of the active-site cleft, even at a low molar excess of the substrate. At elevated substrate concentration laminaritetrose also occupies the active site, spanning the opposite sites +1, +2, +3 and +4 of the cleft
-
?
laminarin + H2O
?
show the reaction diagram
laminarin + H2O
D-glucose + glucooligosaccharides
show the reaction diagram
-
-
if the degree of cleavage of laminarin under exhaustive hydrolysis reaches 74%, the major products are glucose and di-, tri-, and tetrasaccharides
-
?
laminarin + H2O
D-glucose + laminaribiose
show the reaction diagram
-
more specific for compounds having beta-1,3-glucosidic linkages
-
-
?
laminarin + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
CaLam digests laminarin and laminarioligosaccharides except laminaribiose as an endo-beta-1,3-glucanase, releasing glucose, laminaribiose and laminaritriose as the major products
-
-
?
laminarin + H2O
D-glucose + laminarioligosaccharides
show the reaction diagram
laminarin + H2O
glucose + laminaribiose + ?
show the reaction diagram
-
-
-
-
?
laminarin + H2O
glucose + laminaribiose + laminaritriose
show the reaction diagram
laminarin + H2O
laminaribiose + beta-D-glucose
show the reaction diagram
laminarin + H2O
laminaribiose + laminaritriose + laminaritetraose
show the reaction diagram
laminarin + H2O
laminaritriose
show the reaction diagram
preferred substrate
main product
-
?
laminarin + H2O
laminaritriose + ?
show the reaction diagram
-
the mutant enzyme W203F fused to 1,3-beta-D-glucanase catalytic domain shows a 3.6fold increase in specific activity against laminarin as compared with the 1,3-beta-D-glucanase catalytic domain alone with laminaritriose as the major product
major product
-
?
laminarin + H2O
reducing sugar + ?
show the reaction diagram
laminarin from Eisenia bicyclis + H2O
oligoglucosides
show the reaction diagram
-
9% of activity compared to laminarin from Laminaria digitata
-
-
?
laminarin from Laminaria digitata + H2O
oligoglucosides
show the reaction diagram
-
-
mixture with different chain length
-
?
laminarioligosaccharide + H2O
D-glucose + laminaribiose + laminaritriose
show the reaction diagram
CaLam digests laminarin and laminarioligosaccharides except laminaribiose as an endo-beta-1,3-glucanase, releasing glucose, laminaribiose and laminaritriose as the major products
-
-
?
laminarioligosaccharide + H2O
laminaribiose + beta-D-glucose
show the reaction diagram
laminaripentaose + H2O
?
show the reaction diagram
laminaripentaose + H2O
D-glucose + laminaritriose + laminaribiose
show the reaction diagram
-
more specific for compounds having beta-1,3-glucosidic linkages
-
-
?
laminaripentaose + H2O
laminaritriose + laminaribiose
show the reaction diagram
-
-
-
-
?
laminaritetraose + H2O
?
show the reaction diagram
laminaritetraose + H2O
D-glucose + ?
show the reaction diagram
-
more specific for compounds having beta-1,3-glucosidic linkages
-
-
?
laminaritriose + H2O
D-glucose + ?
show the reaction diagram
-
more specific for compounds having beta-1,3-glucosidic linkages
-
-
?
laminaritriose + H2O
D-glucose + laminaribiose
show the reaction diagram
lentinan + H2O
?
show the reaction diagram
4.4% of the activity with laminarin
-
-
?
lichenan + H2O
?
show the reaction diagram
lichenan + H2O
D-glucose + ?
show the reaction diagram
lichenin from Cetraria islandica + H2O
oligoglucosides
show the reaction diagram
-
6% of activity compared to laminarin from Laminaria digitata
-
-
?
mycolaminarin + H2O
reducing sugar
show the reaction diagram
-
highest activity
no glucose release, approximately four glycosidic linkages hydrolyzed per molecule mycolaminarin, predominant product are tetrasaccharides
?
O-carboxymethyl-(1,3:1,6)-beta-D-glucan + H2O
?
show the reaction diagram
O-carboxymethyl-pachyman + H2O
?
show the reaction diagram
oat glucan + H2O
?
show the reaction diagram
-
15% activity compared to laminarin
-
-
?
pachyman + H2O
?
show the reaction diagram
pachyman + H2O
D-glucose + ?
show the reaction diagram
pendulan + H2O
reducing sugar + ?
show the reaction diagram
-
acts in random fashion, reaction product with high molecular weight, major role in decreasing the molecular weight of pendulan during fermentation
-
?
periodate-oxidized laminaran + H2O
?
show the reaction diagram
Littorina kurila
-
95% of the rate with laminaran
-
-
?
periodate-oxidized laminaran + H2O
D-glucose + ?
show the reaction diagram
periodate-oxidized laminarin + H2O
?
show the reaction diagram
polysaccharide + H2O
ethanol-soluble sugar + ?
show the reaction diagram
-
crude fraction from unripe bananas, not active in polysaccharide fraction from ripe bananas, possible involvement in ripening and/or softening process
-
?
pustulan + H2O
?
show the reaction diagram
reduced laminarihexaose + H2O
?
show the reaction diagram
-
-
-
-
?
reduced laminarioligosaccharide + H2O
?
show the reaction diagram
-
19 glucose units
-
-
?
reduced laminarioligosaccharides + H2O
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
show the reaction diagram
-
velocity of catalytic reaction increases with the number of 1,3-beta-glucosidic bonds, preferentially cleaves between the glucose residues at positions 2 and 3 from the reducing end
-
?
schizophyllan + H2O
?
show the reaction diagram
scleroglucan + H2O
?
show the reaction diagram
Sclerotium glucanicum
-
low activity
-
?
short-chain pachyman + H2O
?
show the reaction diagram
-
low affinity
-
?
translam + H2O
?
show the reaction diagram
zymosan A + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alkali soluble 1,3-beta-glucan + H2O
reducing sugar + ?
show the reaction diagram
-
produced by solubilised 1,3-beta-glucan synthase complex of A. fumigatus
-
?
beta-1,3-glucan + H2O
laminaribiose + D-glucose
show the reaction diagram
beta-glucan + H2O
?
show the reaction diagram
callose + H2O
?
show the reaction diagram
Q02126
-
-
-
?
cell wall glucan + H2O
reducing sugar + ?
show the reaction diagram
curdlan + H2O
laminarioligosaccharides + laminaribiose
show the reaction diagram
laminarin + H2O
?
show the reaction diagram
laminarin + H2O
laminaribiose + beta-D-glucose
show the reaction diagram
C6L684
-
-
-
?
laminarioligosaccharide + H2O
laminaribiose + beta-D-glucose
show the reaction diagram
C6L684
substrate specificities and products, overview
-
-
?
pendulan + H2O
reducing sugar + ?
show the reaction diagram
-
acts in random fashion, reaction product with high molecular weight, major role in decreasing the molecular weight of pendulan during fermentation
-
?
polysaccharide + H2O
ethanol-soluble sugar + ?
show the reaction diagram
-
crude fraction from unripe bananas, not active in polysaccharide fraction from ripe bananas, possible involvement in ripening and/or softening process
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
increases activity
Hg2+
-
42% residual activity at 1 mM HgCl2
K3Fe(CN)6
Rhizopus sp.
-
activation on hydrolysis of laminarin, not on lytic activity of living yeast cells
Zn2+
-
slight activation at 2 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2,3)-epoxypropyl beta-D-laminaribiose
-
number of glucosyl residues in the inhibitor affects aglycon chain length specificity and correct positioning of the substrate at the active site
1-ethyl-3(-dimethylaminopropyl)carbodiimide
-
85% residual activity at 10 mM (pH 5.0)
2-Hydroxy-5-nitrobenzylbromide
-
inhibition at 10 mM
2-mercaptoethanol
-
1 mM concentration, activity loss 49%
3,4-epoxybutyl-beta-D-cellobioside
-
-
4-chloromercuribenzoate
carbodiimide
-
inhibition at 100 mM
Co2+
-
slight inhibition at 1 mM
Cr2(SO4)3
-
36% inhibition at 1 mM
Cr3+
loss of 11.6% activity at 5 mM
diethyl dicarbonate
-
complete loss of activity at 36 mM (pH 6.0)
GSSG
-
slight inhibition at 1 mM
Hexadecyltrimethylammonium bromide
Iodine
iodoacetamide
K3Fe(CN)6
Flavobacterium dormitator
-
slight inhibition at 1 mM
KBr
-
slight inhibition at 1 mM
Laminarin
Lespedeza cuneata cellulase inhibitor
-
inhibition at 0.2 mg/ml
-
N-Acetylimidazole
-
inhibition at 23 mM
N-bromosuccinimide
N-ethylmaleimide
-
slight inhibition at 1 mM
Na2WO4
-
36% inhibition at 1 mM
NaAsO2
NaN3
Flavobacterium dormitator
-
slight inhibition at 1 mM
Ni2+
Flavobacterium dormitator
-
slight inhibition at 1 mM
p-hydroxymercuribenzoate
Sclerotium glucanicum
-
strong inhibition at 1 mM
Phenylmercurinitrate
propylene oxide
-
15% residual activity at 6 M (pH 4.0)
Semicarbazide
Flavobacterium dormitator
-
slight inhibition at 1 mM
Sn2+
-
25% inhibition at 1 mM
Sodium 7-deoxycholate
additional information