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Literature summary for 3.2.1.39 extracted from

  • Ilari, A.; Fiorillo, A.; Angelaccio, S.; Florio, R.; Chiaraluce, R.; van der Oost, J.; Consalvi, V.
    Crystal structure of a family 16 endoglucanase from the hyperthermophile Pyrococcus furiosus - structural basis of substrate recognition (2009), FEBS J., 276, 1048-1058.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion technique at 20°C, crystal structure is determined at 2.1 A resolution by molecular replacement Pyrococcus furiosus
to 2.1 A resolution, determination by molecular replacement. Structure reveals a kink of six residues at the entrance of the catalytic cleft Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
delI72-G75 deletion mutant, lacking residues 72-75, hydrolyses the mixed-linkage beta-1,3-1,4-glucan lichenan at 40°C and 70°C 10times more efficiently than the wiild-type protein. At 80°C, the specific activity of the D-loop mutant is reduced significantly relative to that of the wild-type with both laminarin and lichenan Pyrococcus furiosus
additional information deletion mutant lacking resiudes 72-75 hydrolyzes the mixed-linkgae beta-(1-3)-(1-4)-glucan lichenan 10times more efficiently than wild-type Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ one ion per monomer, crystallization data Pyrococcus furiosus
Ca2+ contains one calcium ion for each monomer. The calcium ion is located on the opposite side with respect to the catalytic cleft Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-
Pyrococcus furiosus O73951
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.1
-
wild-type, substrate lichenan, 40°C Pyrococcus furiosus
102.8
-
mutant lacking residues 72-75, substrate lichenan, 80°C Pyrococcus furiosus
115.7
-
mutant lacking residues 72-75, substrate lichenan, 40°C Pyrococcus furiosus
115.7
-
wild-type, substrate lichenan, 70°C Pyrococcus furiosus
126.8
-
wild-type, substrate laminarin, 40°C Pyrococcus furiosus
141.4
-
mutant lacking residues 72-75, substrate laminarin, 40°C Pyrococcus furiosus
185
-
wild-type, substrate lichenan, 80°C Pyrococcus furiosus
249
-
mutant lacking residues 72-75, substrate laminarin, 80°C Pyrococcus furiosus
263.6
-
mutant lacking residues 72-75, substrate lichenan, 70°C Pyrococcus furiosus
334.3
-
mutant lacking residues 72-75, substrate laminarin, 70°C Pyrococcus furiosus
659.3
-
wild-type, substrate laminarin, 70°C Pyrococcus furiosus
1087
-
wild-type, substrate laminarin, 80°C Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O
-
Pyrococcus furiosus ?
-
?
lichenan + H2O
-
Pyrococcus furiosus ?
-
?

Synonyms

Synonyms Comment Organism
PfLamA
-
Pyrococcus furiosus