KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
Laminarin | isoform GI | Hordeum vulgare | |
0.18 | - |
Laminarin | isoform GIII | Hordeum vulgare | |
0.21 | - |
Laminarin | isoform GII | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
isoforms GI, GII and GIII, SDS-PAGE | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
barley | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | isoform GIII, 12-17% carbohydrate content, w/w | Hordeum vulgare |
Purification (Comment) | Organism |
---|---|
to homogeneity, 4-5step chromatography | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40.5 | - |
isoform GIII | Hordeum vulgare |
240.5 | - |
isoform GII | Hordeum vulgare |
281.5 | - |
isoform GI | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-glucan + H2O | low activity | Hordeum vulgare | ? | - |
? | |
beta-glucan + H2O | from yeast | Hordeum vulgare | ? | - |
? | |
laminarin + H2O | highest rates from Laminaria digitata, major products are laminaribiose and laminaritriose for isoforms GII and GIII, GI yields predominantly (1-3)-beta-D-oligoglucosides greater than 4 units | Hordeum vulgare | D-glucose + ? | - |
? | |
O-carboxymethyl-(1,3:1,6)-beta-D-glucan + H2O | from yeast, low activity | Hordeum vulgare | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
(1-3)-beta-D-glucanase | three isoforms, GI, GII and GIII | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
isoforms GI, GII and GIII | Hordeum vulgare |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
below less activity for isoform GI than for GII or GIII | Hordeum vulgare |
6 | - |
isoforms GI and GII retain 17-27% activity, GIII retaines 66% activity | Hordeum vulgare |