Literature summary for 3.2.1.39 extracted from

Wojtkowiak, A.; Witek, K.; Hennig, J.; Jaskolski, M.
Two high-resolution structures of potato endo-1,3-beta-glucanase reveal subdomain flexibility with implications for substrate binding (2012), Acta Crystallogr. Sect. D, 68, 713-723.

Cloned(Commentary)

Cloned (Comment)	Organism
gene gluB20-2, expression in Escherichia coli strain BL21	Solanum tuberosum

Crystallization (Commentary)

Crystallization (Comment)	Organism
two different crystal forms: higher density and lower density determined at 1.40 A and 1.26 A resolution, respectively. The crystal structures reveal an unusual packing mode, clearly visible in the LD structure, caused by the presence of the C-terminal His6 tag, which extends from the compact fold of the enzyme molecule and docks in the catalytic cleft of a neighbouring molecule. In this way, an infinite chain of His-tag-linked protein molecules is formed along the c direction. Purified recombinant His-tagged enzyme by hanging drop vapour diffusion method, mixing of 0.0015 ml of 8 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.0015 ml of reservoir solution containing 0.1 M sodium acetate, pH 4.6, 0.2 M ammonium acetate, 25-30% PEG 4000, 21°C, 2 days, X-ray diffraction structure determination and analysis, modelling	Solanum tuberosum

Crystallization (Comment)

Organism

two different crystal forms: higher density and lower density determined at 1.40 A and 1.26 A resolution, respectively. The crystal structures reveal an unusual packing mode, clearly visible in the LD structure, caused by the presence of the C-terminal His6 tag, which extends from the compact fold of the enzyme molecule and docks in the catalytic cleft of a neighbouring molecule. In this way, an infinite chain of His-tag-linked protein molecules is formed along the c direction. Purified recombinant His-tagged enzyme by hanging drop vapour diffusion method, mixing of 0.0015 ml of 8 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.0015 ml of reservoir solution containing 0.1 M sodium acetate, pH 4.6, 0.2 M ammonium acetate, 25-30% PEG 4000, 21°C, 2 days, X-ray diffraction structure determination and analysis, modelling

Solanum tuberosum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	the enzyme has a 23-residue signal peptide at the N-terminus	Solanum tuberosum	-	-

Organism

Organism	UniProt	Comment	Textmining
Solanum tuberosum	Q70C53	gene gluB20-2	-
Solanum tuberosum Desiree	Q70C53	gene gluB20-2	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli strain BL21 cell-free extract by ammonium sulfate fractionation, anion exchange chromatography, nickel affinity chromatography, and dialysis	Solanum tuberosum

Synonyms

Synonyms	Comment	Organism
endo-1,3-beta-glucanase	-	Solanum tuberosum

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the glycosyl hydrolase family 17, GH17	Solanum tuberosum