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Literature summary for 3.2.1.39 extracted from
- Purification and properties of lytic beta-1,3-glucanase from Flavobacterium dormitator var. glucanolyticae (1976), Agric. Biol. Chem., 40, 1059-1067.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Sodium diethyldithiocarbamate | 10% activation at 1 mM | Flavobacterium dormitator |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 45% inhibition at 1 mM | Flavobacterium dormitator | |
| Cu2+ | strong inhibition at 1 mM | Flavobacterium dormitator | |
| EDTA | nearly complete inhibition at 1 mM | Flavobacterium dormitator | |
| Hg2+ | 36% inhibition at 1 mM | Flavobacterium dormitator | |
| Iodine | 30% inhibition at 1 mM | Flavobacterium dormitator | |
| K3Fe(CN)6 | slight inhibition at 1 mM | Flavobacterium dormitator | |
| KMnO4 | complete inhibition at 1 mM | Flavobacterium dormitator | |
| NaAsO2 | slight inhibition at 1 mM | Flavobacterium dormitator | |
| NaN3 | slight inhibition at 1 mM | Flavobacterium dormitator | |
| Ni2+ | slight inhibition at 1 mM | Flavobacterium dormitator | |
| Pb2+ | 59% inhibition at 1 mM | Flavobacterium dormitator | |
| Semicarbazide | slight inhibition at 1 mM | Flavobacterium dormitator | |
| Zn2+ | 25% inhibition at 1 mM | Flavobacterium dormitator | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Laminarin | value in mg/ml | Flavobacterium dormitator | |
| additional information | - |
Glucan | value in mg/ml: 1.8 | Flavobacterium dormitator | |
| additional information | - |
Laminarihexaose | value in mg/ml: 1.18 | Flavobacterium dormitator | |
| additional information | - |
laminaritetraose | value in mg/ml: 2.0 | Flavobacterium dormitator | |
| additional information | - |
Laminarin | 0.26 | Flavobacterium dormitator | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Flavobacterium dormitator | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 15% activation at 1 mM | Flavobacterium dormitator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | 20000 | SDS-PAGE, gel filtration | Flavobacterium dormitator |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell wall glucan + H2O | Flavobacterium dormitator | living yeast cells | reducing sugar + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Flavobacterium dormitator | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Flavobacterium dormitator |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Flavobacterium dormitator | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.1 | - |
- |
Flavobacterium dormitator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1-3:1-4)-beta-D-glucan + H2O | lichenan | Flavobacterium dormitator | ? | - |
? | |
| 3-O-beta-D-Glc-D-Glc-D-Glc + H2O | releases laminaribiose and glucose | Flavobacterium dormitator | ? | - |
? | |
| 3-O-beta-D-Glc-D-Glc-D-Glc + H2O | laminaritriose | Flavobacterium dormitator | ? | - |
? | |
| 3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Flavobacterium dormitator | beta-D-glucose + D-glucose | - |
? | |
| beta-D-glucan + H2O | - |
Flavobacterium dormitator | ? | - |
? | |
| beta-glucan + H2O | from yeast | Flavobacterium dormitator | ? | - |
? | |
| cell wall glucan + H2O | living yeast cells | Flavobacterium dormitator | reducing sugar + ? | - |
? | |
| laminarihexaose + H2O | - |
Flavobacterium dormitator | ? | - |
? | |
| laminarin + H2O | major products are glucose, laminaribiose, laminaritriose, laminaritetraose and higher liminarioligosaccharides | Flavobacterium dormitator | D-glucose + ? | - |
? | |
| pachyman + H2O | - |
Flavobacterium dormitator | ? | - |
? | |
| periodate-oxidized laminarin + H2O | - |
Flavobacterium dormitator | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Flavobacterium dormitator |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
complete loss of activity above | Flavobacterium dormitator |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Flavobacterium dormitator |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | stable within | Flavobacterium dormitator |
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