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Literature summary for 3.2.1.39 extracted from
- beta-1,3-Endoglucanase from soybean releases elicitor-active carbohydrates from fungus cell walls (1983), Plant Physiol., 71, 460-465.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
pH 10.7 isoform, SDS-PAGE, gel filtration | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell wall glucan + H2O | Glycine max | from Phytophthora megasperma, carbohydrate release to elicit glyceollin accumulation in cotyledons | reducing sugar + ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
soybean | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 2step chromatography, isoelectric focusing | Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.6 | - |
pH10.7-isoform | Glycine max |
Storage Stability
| Storage Stability | Organism |
|---|---|
| retains full activity following lyophilization for 3 months if stored at -20°C, in solution stable if stored at -20°C | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell wall glucan + H2O | from Phytophthora megasperma, carbohydrate release to elicit glyceollin accumulation in cotyledons | Glycine max | reducing sugar + ? | - |
? | |
| laminarin + H2O | lower activity than on mycolaminarin | Glycine max | D-glucose + ? | - |
? | |
| mycolaminarin + H2O | highest activity | Glycine max | reducing sugar | no glucose release, approximately four glycosidic linkages hydrolyzed per molecule mycolaminarin, predominant product are tetrasaccharides | ? | |
| O-carboxymethyl-pachyman + H2O | - |
Glycine max | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3-endoglucanase | two isoforms, pH 8.7 and pH 10.5 glucanases | Glycine max |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
inactivation above | Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
- |
Glycine max |
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Release 2023.1 (January 2023)
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