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Literature summary for 3.2.1.39 extracted from

  • Reichelt, B.Y.; Fleet, G.H.
    Isolation, properties, function, and regulation of endo-(1-3)-beta-glucanases in Schizosaccharomyces pombe (1981), J. Bacteriol., 147, 1085-1094.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.39

Activating Compound

Activating Compound Comment Organism Structure
Trypsin stimulation of release from cell walls Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
Laminarin slight inhibition above 1 mg/ml for glucanase I, above 0.5 mg/ml inhibitory for glucanase II Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 Laminarin value in mg/ml Schizosaccharomyces pombe
additional information
-
 Laminarin 0.15 , glucanase I Schizosaccharomyces pombe
additional information
-
 Laminarin 0.69 glucanase II Schizosaccharomyces pombe

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall two different forms, only present in conjugating and sporulating cells, not present in vegetative cells Schizosaccharomyces pombe 5618
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 SDS-PAGE, gel filtration, glucanase II Schizosaccharomyces pombe
78500
-
 1 * 78500, 1 * 82000, glucanase II, SDS-PAGE Schizosaccharomyces pombe
82000
-
 1 * 78500, 1 * 82000, glucanase II, SDS-PAGE Schizosaccharomyces pombe
160500
-
 SDS-PAGE, gel filtration, glucanase I Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cell wall glucan + H2O Schizosaccharomyces pombe involved in modification and limited degradation of the cell wall during cell life cycle reducing sugar + ?
-
 ?
cell wall glucan + H2O Schizosaccharomyces pombe cell wall autolysis reducing sugar + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, 3step chromatography for glucanase I, 4step chromatography for glucanase II Schizosaccharomyces pombe

Source Tissue

Source Tissue Comment Organism Textmining
cell culture enzyme activation due to factors produced during conjugation and sporulation Schizosaccharomyces pombe
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.89
-
 for glucanase I Schizosaccharomyces pombe
5.89
-
 for glucanase II Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cell wall glucan + H2O involved in modification and limited degradation of the cell wall during cell life cycle Schizosaccharomyces pombe reducing sugar + ?
-
 ?
cell wall glucan + H2O cell wall autolysis Schizosaccharomyces pombe reducing sugar + ?
-
 ?
laminarin + H2O specifically hydrolyzes (1-3)-beta-glycosidic linkages, predominant products are laminaribiose and laminaritriose, minor amounts of glucose and higher oligosaccharides Schizosaccharomyces pombe D-glucose + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 78500, 1 * 82000, glucanase II, SDS-PAGE Schizosaccharomyces pombe
monomer 1 * 75000, glucanase I, SDS-PAGE, gel filtration Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
endo-(1-3)-beta-glucanase two different forms, glucanase I and glucanase II Schizosaccharomyces pombe

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 both glucanases I and II Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 both forms, glucanase I and glucanase II Schizosaccharomyces pombe