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Literature summary for 3.2.1.39 extracted from
- Characterization of the endo-beta-1,3-glucanase activity of S. cerevisiae Eng2 and other members of the GH81 family (2008), Fungal Genet. Biol., 45, 542-553.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D518A | no activity | Saccharomyces cerevisiae |
| D526A | enzymatic activity is similar to that of the wild type protein | Saccharomyces cerevisiae |
| D545A | enzymatic activity is similar to that of the wild type protein | Saccharomyces cerevisiae |
| D570A | no activity | Saccharomyces cerevisiae |
| D588A | no activity | Saccharomyces cerevisiae |
| E609A | no activity | Saccharomyces cerevisiae |
| E613A | no activity | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 0.1 mM | Saccharomyces cerevisiae |  |
| Hg2+ | 0.1 mM | Schizosaccharomyces pombe | |
| additional information | Ca2+, Cu2+,Fe3+, Mg2+, permanganate, and N-Br-succinimide have no effect on enzymatic activity | Saccharomyces cerevisiae | |
| additional information | Ca2+, Cu2+, Fe3+, Mg2+, permanganate, and N-Br-succinimide have no effect on enzymatic activity | Schizosaccharomyces pombe |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae YPA84 | - |
- |
- |
| Schizosaccharomyces pombe | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
| CM-Sepharose Fast Flow column chromatography | Schizosaccharomyces pombe |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 37°C, 1 h, no loss of activity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | - |
Saccharomyces cerevisiae | laminaribiose + laminaritriose + laminaritetraose | main reaction products | ? | |
| laminarin + H2O | - |
Schizosaccharomyces pombe | laminaribiose + laminaritriose + laminaritetraose | main reaction products | ? | |
| laminarin + H2O | - |
Saccharomyces cerevisiae YPA84 | laminaribiose + laminaritriose + laminaritetraose | main reaction products | ? | |
| additional information | laminaribiose and laminaritriose are no substrates, laminaritetraose is very inefficiently cleaved | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | laminaribiose and laminaritriose are no substrates, laminaritetraose is very inefficiently cleaved | Schizosaccharomyces pombe | ? | - |
? | |
| additional information | laminaribiose and laminaritriose are no substrates, laminaritetraose is very inefficiently cleaved | Saccharomyces cerevisiae YPA84 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,3-glucanase | - |
Saccharomyces cerevisiae |
| endo-beta-1,3-glucanase | - |
Schizosaccharomyces pombe |
| Eng2 protein | - |
Saccharomyces cerevisiae |
| Eng2 protein | - |
Schizosaccharomyces pombe |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
Schizosaccharomyces pombe |
| 55 | - |
- |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Schizosaccharomyces pombe |
| 6.5 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | - |
Schizosaccharomyces pombe |
| 5.5 | 8.5 | - |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
activity decreases rapidly at pH lower 4.5 | Schizosaccharomyces pombe |
| 5.5 | - |
activity decreases rapidly at pH lower 5.5 | Saccharomyces cerevisiae |
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