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Literature summary for 3.2.1.39 extracted from
- Lysis of yeast cell walls (1976), Eur. J. Biochem., 63, 121-130.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
Laminarin | value in mg/ml | Niallia circulans |  |
| additional information | - |
Laminarin | 1.05 lytic beta-(1-3)-glucanase I | Niallia circulans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Niallia circulans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
SDS-PAGE, gel filtration | Niallia circulans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cell wall glucan + H2O | Niallia circulans | - |
reducing sugar + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by absortion chromatography for lytic beta-(1-3)-glucanase I, partial for lytic beta-(1-3)-glucanase II | Niallia circulans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | induction of enzyme synthesis by glucan culture medium | Niallia circulans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.1 | - |
lytic beta-(1-3)-glucanase I | Niallia circulans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -25°C, 2 mM sodium succinate, pH 6.0, two months | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-O-beta-D-Glc-D-Glc-D-Glc + H2O | releases laminaribiose and glucose | Niallia circulans | ? | - |
? | |
| 3-O-beta-D-Glc-D-Glc-D-Glc + H2O | laminaritriose | Niallia circulans | ? | - |
? | |
| beta-glucan + H2O | from yeast | Niallia circulans | ? | - |
? | |
| beta-glucan + H2O | from oat | Niallia circulans | ? | - |
? | |
| cell wall glucan + H2O | - |
Niallia circulans | reducing sugar + ? | - |
? | |
| laminarin + H2O | end-products are laminarihexaose to laminaritriose, some glucose and little gentiobiose for lytic beta-(1-3)-glucanase I | Niallia circulans | D-glucose + ? | - |
? | |
| pachyman + H2O | - |
Niallia circulans | ? | - |
? | |
| periodate-oxidized laminarin + H2O | - |
Niallia circulans | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lytic beta-(1-3)-glucanase I | - |
Niallia circulans |
| lytic beta-(1-3)-glucanase II | - |
Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Niallia circulans |
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Release 2023.1 (January 2023)
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