Protein Variants | Comment | Organism |
---|---|---|
E231A | no enzymic activity with laminarin, in presence of sodium formate, recovery of 75% of activitiy compared to wild type, with substrate alpha-laminaribiosyl fluoride, polymerization to insoluble, crystalline (1,3)-beta-D-glucans | Hordeum vulgare |
E231G | no enzymic activity with laminarin, in presence of sodium formate, recovery of 75% of activitiy compared to wild type, with substrate alpha-laminaribiosyl fluoride, polymerization to insoluble, crystalline (1,3)-beta-D-glucans | Hordeum vulgare |
E231S | no enzymic activity with laminarin, with substrate alpha-laminaribiosyl fluoride, polymerization to insoluble, crystalline (1,3)-beta-D-glucans | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.7 | - |
alpha-laminaribiosyl fluoride | pH 6.2, 30°C, mutant E231G | Hordeum vulgare | |
19.8 | - |
alpha-laminaribiosyl fluoride | pH 6.2, 30°C, mutant E231S | Hordeum vulgare | |
25.9 | - |
alpha-laminaribiosyl fluoride | pH 6.2, 30°C, mutant E231A | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
barley | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
220 | - |
pH 6.2, 30°C | Hordeum vulgare |
Storage Stability | Organism |
---|---|
4°C, 50 mM sodium phosphate, pH 7.8, 300 mM NaCl, stable for several weeks | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-laminaribiosyl fluoride + H2O | - |
Hordeum vulgare | ? | - |
? | |
laminarin + H2O | - |
Hordeum vulgare | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
wild-type | Hordeum vulgare |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | broad, mutant E231G | Hordeum vulgare |