EC Number |
Protein Variants |
Reference |
---|
3.2.1.39 | C6A |
significant decrease in activity towards carboxymethyl-curdlan and laminarin, strong increase in activity towards lichenan |
695310 |
3.2.1.39 | D287A |
decreased activity |
679797 |
3.2.1.39 | D422A |
site-directed mutagenesis, the mutant enzyme shows altered activity with polysaccharide substrates compared with the wild-type enzyme |
-, 710465 |
3.2.1.39 | D422E |
site-directed mutagenesis, the mutant enzyme shows altered activity with polysaccharide substrates compared with the wild-type enzyme |
710465 |
3.2.1.39 | D424A |
site-directed mutagenesis, the mutant enzyme shows altered activity with polysaccharide substrates compared with the wild-type enzyme |
-, 710465 |
3.2.1.39 | D424H |
site-directed mutagenesis, the mutation alters the substrate specificity by increasing the rate of cleavage of mixed-linkage beta-glucan and carboxymethylcellulose 60fold and 16fold, respectively, compared to the wild-type enzyme |
-, 710465 |
3.2.1.39 | D424H/S501A |
site-directed mutagenesis, the mutant enzyme shows altered activity with polysaccharide substrates compared with the wild-type enzyme |
710465 |
3.2.1.39 | D518A |
no activity |
-, 679981 |
3.2.1.39 | D526A |
enzymatic activity is similar to that of the wild type protein |
-, 679981 |
3.2.1.39 | D545A |
enzymatic activity is similar to that of the wild type protein |
679981 |