Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
D287A | decreased activity | Pyrococcus furiosus |
E53A | decreased activity | Pyrococcus furiosus |
E53A/D287A | decreased activity | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Renatured (Comment) | Organism |
---|---|
refolding is started by 20fold dilution of the unfolding mixture, at 20°C, into solutions of buffer containing decreasing guanidinium chloride concentrations | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-glucan + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
laminarin + H2O | - |
Pyrococcus furiosus | D-glucose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,3-glucanase | - |
Pyrococcus furiosus |