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Literature summary for 3.2.1.39 extracted from

  • Blaettel, V.; Larisika, M.; Pfeiffer, P.; Nowak, C.; Eich, A.; Eckelt, J.; Koenig, H.
    Beta-1,3-glucanase from Delftia tsuruhatensis strain MV01 and its potential application in vinification (2011), Appl. Environ. Microbiol., 77, 983-990.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.39

Application

Application Comment Organism
food industry enzyme is able to hydrolyze both polymers, the beta-1,3-glucan from wine-related lactic acid bacterium Pediococcus parvus and that from yeast cell walls, which can make wine filtration difficult or impossible. Enzyme is still active under wine-relevant parameters such as elevated ethanol, sulfite, and phenol concentrations as well as at low pH values. Enzyme seems to be a useful tool to prevent slime production and undesirable yeast growth during vinification Delftia tsuruhatensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, SDS-PAGE Delftia tsuruhatensis

Organism

Organism UniProt Comment Textmining
Delftia tsuruhatensis
-
-
-
Delftia tsuruhatensis MV01
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Delftia tsuruhatensis

Storage Stability

Storage Stability Organism
20°C, 48 h, 75% residual activity Delftia tsuruhatensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-glucan + H2O sources: Pediococcus parvalus and yeast Delftia tsuruhatensis ?
-
 ?
beta-1,3-glucan + H2O sources: Pediococcus parvalus and yeast Delftia tsuruhatensis MV01 ?
-
 ?
carboxymethyl pachyman + H2O 135% of the activity with laminarin Delftia tsuruhatensis ?
-
 ?
carboxymethyl pachyman + H2O 135% of the activity with laminarin Delftia tsuruhatensis MV01 ?
-
 ?
laminarin + H2O
-
 Delftia tsuruhatensis D-glucose + laminarioligosaccharides
-
 ?
laminarin + H2O
-
 Delftia tsuruhatensis MV01 D-glucose + laminarioligosaccharides
-
 ?
additional information enzyme cleaves cell wall debris of yeast Pichia fermentans, Zygosaccharomyces bailii, Saccharomycodes ludwigii, Dekkera bruxellensis. No substrate: xylan, pustulan, carboxymethyl cellulose, dextran Delftia tsuruhatensis ?
-
 ?
additional information enzyme cleaves cell wall debris of yeast Pichia fermentans, Zygosaccharomyces bailii, Saccharomycodes ludwigii, Dekkera bruxellensis. No substrate: xylan, pustulan, carboxymethyl cellulose, dextran Delftia tsuruhatensis MV01 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Delftia tsuruhatensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Delftia tsuruhatensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 80
-
 Delftia tsuruhatensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 24 h, 17% residual activity Delftia tsuruhatensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Delftia tsuruhatensis

pH Range

pH Minimum pH Maximum Comment Organism
3 8
-
 Delftia tsuruhatensis

pI Value

Organism Comment pI Value Maximum pI Value
Delftia tsuruhatensis isoelectric focusing
-
 4.2