Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 9,6-10% activation at 1-5 mM | Streptomyces sp. |
Application | Comment | Organism |
---|---|---|
agriculture | BglS27 is a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation | Streptomyces sp. |
biotechnology | BglS27 is a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation | Streptomyces sp. |
food industry | BglS27 is a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation | Streptomyces sp. |
Cloned (Comment) | Organism |
---|---|
gene bglS27, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | loss of 11.5% activity at 5 mM | Streptomyces sp. | |
Cr3+ | loss of 11.6% activity at 5 mM | Streptomyces sp. | |
Cu2+ | loss of 24.4% activity at 5 mM | Streptomyces sp. | |
EDTA | - |
Streptomyces sp. | |
Fe2+ | loss of 22.6% activity at 5 mM | Streptomyces sp. | |
Mn2+ | loss of 24.2% activity at 5 mM | Streptomyces sp. | |
Zn2+ | loss of 22.0% activity at 5 mM | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 1.89 mg/ml for laminarin, recombinant enzyme | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 14.1% activation at 1 mM, 30% at 5 mM | Streptomyces sp. | |
Mn2+ | 20.4% activation at 1 mM | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42700 | - |
x * 42700, about, sequence calculation | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | Streptomyces sp. | - |
? | - |
? | |
additional information | Streptomyces sp. | BglS27 preferentially catalyzes the hydrolysis of glucans with a beta-1,3-linkage using an endolytic mode of action | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | C3VPJ1 | gene bglS27 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged BglS27 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Streptomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
236 | - |
purified recombinant enzyme, substrate laminarin | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-glucan + H2O | Barley beta-glucan, 1,3-1,4-beta-glucose monomers, high activity | Streptomyces sp. | ? | - |
? | |
laminarin + H2O | - |
Streptomyces sp. | ? | - |
? | |
laminarin + H2O | 1,3-beta-glucose monomers, best substrate | Streptomyces sp. | ? | - |
? | |
lichenan + H2O | 1,3-1,4-beta-glucose monomers, high activity | Streptomyces sp. | ? | - |
? | |
additional information | BglS27 preferentially catalyzes the hydrolysis of glucans with a beta-1,3-linkage using an endolytic mode of action | Streptomyces sp. | ? | - |
? | |
additional information | no activity with oat spelt xylan and carboxymethyl cellulose | Streptomyces sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42700, about, sequence calculation | Streptomyces sp. |
More | the encoded protein comprises a catalytic module of glycosyl hydrolase family 16, a short glycine linker region, and a family 13 carbohydrate-binding module | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
BglS27 | - |
Streptomyces sp. |
endo-1,3-beta-D-glucanase | - |
Streptomyces sp. |
More | the bacterial enzyme belongs to glycosylhydrolase family GH16 | Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
recombinant enzyme | Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant enzyme, over 80% activity remaining after 30 min | Streptomyces sp. |
70 | 80 | purified recombinant enzyme, over 40% activity remaining after 30 min | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant enzyme | Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity range | Streptomyces sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | BglS27 has the ability to inhibit the growth of phytopathogenic fungi Rhizoctonic solani and Fusarium oxysporum and some mycotoxin-producing fungi Fusarium crookwellense and Paecilomyces variotii | Streptomyces sp. |