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Literature summary for 3.2.1.39 extracted from

  • Shi, P.; Yao, G.; Yang, P.; Li, N.; Luo, H.; Bai, Y.; Wang, Y.; Yao, B.
    Cloning, characterization, and antifungal activity of an endo-1,3-beta-D-glucanase from Streptomyces sp. S27 (2010), Appl. Microbiol. Biotechnol., 85, 1483-1490.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 9,6-10% activation at 1-5 mM Streptomyces sp.

Application

Application Comment Organism
agriculture BglS27 is a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation Streptomyces sp.
biotechnology BglS27 is a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation Streptomyces sp.
food industry BglS27 is a good candidate for utilization in biotechnological applications such as plant protection, feed, and food preservation Streptomyces sp.

Cloned(Commentary)

Cloned (Comment) Organism
gene bglS27, DNA and amino acid sequence determination and analysis, expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ loss of 11.5% activity at 5 mM Streptomyces sp.
Cr3+ loss of 11.6% activity at 5 mM Streptomyces sp.
Cu2+ loss of 24.4% activity at 5 mM Streptomyces sp.
EDTA
-
Streptomyces sp.
Fe2+ loss of 22.6% activity at 5 mM Streptomyces sp.
Mn2+ loss of 24.2% activity at 5 mM Streptomyces sp.
Zn2+ loss of 22.0% activity at 5 mM Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 1.89 mg/ml for laminarin, recombinant enzyme Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 14.1% activation at 1 mM, 30% at 5 mM Streptomyces sp.
Mn2+ 20.4% activation at 1 mM Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42700
-
x * 42700, about, sequence calculation Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
laminarin + H2O Streptomyces sp.
-
?
-
?
additional information Streptomyces sp. BglS27 preferentially catalyzes the hydrolysis of glucans with a beta-1,3-linkage using an endolytic mode of action ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. C3VPJ1 gene bglS27
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged BglS27 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Streptomyces sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
236
-
purified recombinant enzyme, substrate laminarin Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + H2O Barley beta-glucan, 1,3-1,4-beta-glucose monomers, high activity Streptomyces sp. ?
-
?
laminarin + H2O
-
Streptomyces sp. ?
-
?
laminarin + H2O 1,3-beta-glucose monomers, best substrate Streptomyces sp. ?
-
?
lichenan + H2O 1,3-1,4-beta-glucose monomers, high activity Streptomyces sp. ?
-
?
additional information BglS27 preferentially catalyzes the hydrolysis of glucans with a beta-1,3-linkage using an endolytic mode of action Streptomyces sp. ?
-
?
additional information no activity with oat spelt xylan and carboxymethyl cellulose Streptomyces sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 42700, about, sequence calculation Streptomyces sp.
More the encoded protein comprises a catalytic module of glycosyl hydrolase family 16, a short glycine linker region, and a family 13 carbohydrate-binding module Streptomyces sp.

Synonyms

Synonyms Comment Organism
BglS27
-
Streptomyces sp.
endo-1,3-beta-D-glucanase
-
Streptomyces sp.
More the bacterial enzyme belongs to glycosylhydrolase family GH16 Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
recombinant enzyme Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified recombinant enzyme, over 80% activity remaining after 30 min Streptomyces sp.
70 80 purified recombinant enzyme, over 40% activity remaining after 30 min Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
recombinant enzyme Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9 activity range Streptomyces sp.

General Information

General Information Comment Organism
physiological function BglS27 has the ability to inhibit the growth of phytopathogenic fungi Rhizoctonic solani and Fusarium oxysporum and some mycotoxin-producing fungi Fusarium crookwellense and Paecilomyces variotii Streptomyces sp.