Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain B834(DE3) | Nocardiopsis sp. |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method with 0.1 M Tris-HCl (pH 8.0), 1.5 M ammonium sulfate, 0.1 M sodium chloride, and 1% (v/v) ethanol | Nocardiopsis sp. |
Protein Variants | Comment | Organism |
---|---|---|
W118A | the mutant almost abolishes its activity | Nocardiopsis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-epoxybutyl-beta-D-cellobioside | - |
Nocardiopsis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | one calcium ion is bound on the convex face of the protein, suggesting to play a role in stabilizing the protein structure | Nocardiopsis sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardiopsis sp. | - |
- |
- |
Nocardiopsis sp. F96 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Nocardiopsis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | - |
Nocardiopsis sp. | D-glucose + ? | - |
? | |
laminarin + H2O | - |
Nocardiopsis sp. F96 | D-glucose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,3-glucanase | - |
Nocardiopsis sp. |