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Literature summary for 3.2.1.39 extracted from
- Molecular characterization of endo-1,3-beta-glucanase from Cellulosimicrobium cellulans: Effects of carbohydrate-binding module on enzymatic function and stability (2011), Biochim. Biophys. Acta, 1814, 1713-1719.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of catalytic domain Ala1-Thr256 in Escherichia coli | Cellulosimicrobium cellulans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E119A | variant of catalytic domain, significant decrease in hydrolytic activity for laminarin | Cellulosimicrobium cellulans |
| E119A/Ldel | variant of catalytic domain with deletion of the Gly/Ser-rich linker, Ser244-Thr256, no expression of protein | Cellulosimicrobium cellulans |
| M123del | variant of catalytic domain, significant decrease in hydrolytic activity for laminarin | Cellulosimicrobium cellulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
Laminariheptaose | recombinant catalytic domain, pH 7.0, 25°C | Cellulosimicrobium cellulans |  |
| 0.32 | - |
Laminaripentaose | native enzyme, pH 7.0, 25°C | Cellulosimicrobium cellulans | |
| 0.36 | - |
Laminaripentaose | recombinant catalytic domain, pH 7.0, 25°C | Cellulosimicrobium cellulans | |
| 0.75 | - |
Laminariheptaose | native enzyme, pH 7.0, 25°C | Cellulosimicrobium cellulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40798 | - |
x * 41000, SDS-PAGE, x * 40798, MALDI-TOF | Cellulosimicrobium cellulans |
| 41000 | - |
x * 41000, SDS-PAGE, x * 40798, MALDI-TOF | Cellulosimicrobium cellulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellulosimicrobium cellulans | - |
enzyme comprises a catalytic domain of the glycoside hydrolase family 16 and a C-terminal carbohydrate-binding module family 13 | - |
| Cellulosimicrobium cellulans DK-1 | - |
enzyme comprises a catalytic domain of the glycoside hydrolase family 16 and a C-terminal carbohydrate-binding module family 13 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both native enzyme and recombinant catalytic domain | Cellulosimicrobium cellulans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | Tunicase, a crude enzyme preparation from Cellulosimicrobium cellulans DK-1 | Cellulosimicrobium cellulans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant catalytic domain, pH 7.0, 25°C | Cellulosimicrobium cellulans |
| 8.47 | - |
native enzyme, pH 7.0, 25°C | Cellulosimicrobium cellulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminariheptaose + H2O | - |
Cellulosimicrobium cellulans | ? | - |
? | |
| laminariheptaose + H2O | - |
Cellulosimicrobium cellulans DK-1 | ? | - |
? | |
| laminarin + H2O | - |
Cellulosimicrobium cellulans | ? | - |
? | |
| laminarin + H2O | - |
Cellulosimicrobium cellulans DK-1 | ? | - |
? | |
| laminaripentaose + H2O | - |
Cellulosimicrobium cellulans | ? | - |
? | |
| laminaripentaose + H2O | - |
Cellulosimicrobium cellulans DK-1 | ? | - |
? | |
| additional information | no substrate: laminaribiose or laminaritriose, carboxymethyl cellulose, chitin, and pustulan | Cellulosimicrobium cellulans | ? | - |
? | |
| additional information | no substrate: laminaribiose or laminaritriose, carboxymethyl cellulose, chitin, and pustulan | Cellulosimicrobium cellulans DK-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, SDS-PAGE, x * 40798, MALDI-TOF | Cellulosimicrobium cellulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, complete loss of activity both for catalytic domain and native enzyme | Cellulosimicrobium cellulans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 30 | - |
Laminariheptaose | recombinant catalytic domain, pH 7.0, 25°C | Cellulosimicrobium cellulans | |
| 42 | - |
Laminaripentaose | recombinant catalytic domain, pH 7.0, 25°C | Cellulosimicrobium cellulans | |
| 69 | - |
Laminariheptaose | native enzyme, pH 7.0, 25°C | Cellulosimicrobium cellulans | |
| 75 | - |
Laminaripentaose | native enzyme, pH 7.0, 25°C | Cellulosimicrobium cellulans | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | native enzyme | Cellulosimicrobium cellulans |
| 4 | - |
loss of activity below, recombinant catalytic domain | Cellulosimicrobium cellulans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 93 | - |
Laminariheptaose | native enzyme, pH 7.0, 25°C | Cellulosimicrobium cellulans | |
| 118 | - |
Laminaripentaose | recombinant catalytic domain, pH 7.0, 25°C | Cellulosimicrobium cellulans | |
| 156 | - |
Laminariheptaose | recombinant catalytic domain, pH 7.0, 25°C | Cellulosimicrobium cellulans | |
| 237 | - |
Laminaripentaose | native enzyme, pH 7.0, 25°C | Cellulosimicrobium cellulans | |
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