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Literature summary for 3.2.1.39 extracted from

  • Archambault, C.; Coloccia, G.; Kermasha, S.; Jabaji-Hare, S.
    Characterization of an endo-1,3-beta-D-glucanase produced during the interaction between the mycoparasite Stachybotry elegans and its host Rhizoctonia solani (1998), Can. J. Microbiol., 44, 989-997.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
Laminarin value in mg/ml Stachybotrys elegans
additional information
-
Laminarin 0.02 Stachybotrys elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Stachybotrys elegans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000 79000 gel filtration, native PAGE, SDS-PAGE Stachybotrys elegans

Organism

Organism UniProt Comment Textmining
Stachybotrys elegans
-
grown on purified cell wall of Rhizotonia solani
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography, preparative gel electrophoresis Stachybotrys elegans

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Stachybotrys elegans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
-
Stachybotrys elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose, low activity Stachybotrys elegans beta-D-glucose + D-glucose
-
?
beta-glucan + H2O 31.2% activity Stachybotrys elegans ?
-
?
laminarin + H2O very active against substrates containing beta-1,3-linkages, mainly disaccharides and oligosaccharides produced, involvement in mycoparasitism Stachybotrys elegans D-glucose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 75000-79000, gel filtration, SDS-PAGE Stachybotrys elegans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Stachybotrys elegans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 50
-
Stachybotrys elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Stachybotrys elegans