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Literature summary for 3.2.1.39 extracted from

  • Song, J.M.; Nam, K.; Sun, Y.U.; Kang, M.H.; Kim, C.G.; Kwon, S.T.; Lee, J.; Lee, Y.H.
    Molecular and biochemical characterizations of a novel arthropod endo-beta-1,3-glucanase from the Antarctic springtail, Cryptopygus antarcticus, horizontally acquired from bacteria (2010), Comp. Biochem. Physiol. B, 155, 403-412.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene CaLam, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic analysis and tree, recombinant expression of His-tagged and thioredoxin-fused enzyme without signal sequence in Escherichia coli strain Rosetta(DE3), subcloning in Escherichia coli strain DH5alpha Cryptopygus antarcticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Cryptopygus antarcticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28983
-
x * 28983, sequence calculation, x * 46000, recombinant His-tagged and thioredoxin-fused enzyme, SDS-PAGE Cryptopygus antarcticus
46000
-
x * 28983, sequence calculation, x * 46000, recombinant His-tagged and thioredoxin-fused enzyme, SDS-PAGE Cryptopygus antarcticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
laminarin + H2O Cryptopygus antarcticus
-
?
-
?

Organism

Organism UniProt Comment Textmining
Cryptopygus antarcticus C1IE32 gene CaLam
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminarin + H2O
-
Cryptopygus antarcticus ?
-
?
laminarin + H2O CaLam digests laminarin and laminarioligosaccharides except laminaribiose as an endo-beta-1,3-glucanase, releasing glucose, laminaribiose and laminaritriose as the major products Cryptopygus antarcticus D-glucose + laminaribiose + laminaritriose
-
?
laminarioligosaccharide + H2O CaLam digests laminarin and laminarioligosaccharides except laminaribiose as an endo-beta-1,3-glucanase, releasing glucose, laminaribiose and laminaritriose as the major products Cryptopygus antarcticus D-glucose + laminaribiose + laminaritriose
-
?

Subunits

Subunits Comment Organism
? x * 28983, sequence calculation, x * 46000, recombinant His-tagged and thioredoxin-fused enzyme, SDS-PAGE Cryptopygus antarcticus
More the enzyme contains a putative signal peptide and a typical motif of glycosyl hydrolase family 16, EIDITE, CaLam homology modelling, overview Cryptopygus antarcticus

Synonyms

Synonyms Comment Organism
endo-beta-1,3-glucanase
-
Cryptopygus antarcticus
More the enzyme belongs to the glycosyl hydrolase family 16, GH16 Cryptopygus antarcticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Cryptopygus antarcticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Cryptopygus antarcticus