Cloned (Comment) | Organism |
---|---|
gene CaLam, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic analysis and tree, recombinant expression of His-tagged and thioredoxin-fused enzyme without signal sequence in Escherichia coli strain Rosetta(DE3), subcloning in Escherichia coli strain DH5alpha | Cryptopygus antarcticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Cryptopygus antarcticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28983 | - |
x * 28983, sequence calculation, x * 46000, recombinant His-tagged and thioredoxin-fused enzyme, SDS-PAGE | Cryptopygus antarcticus |
46000 | - |
x * 28983, sequence calculation, x * 46000, recombinant His-tagged and thioredoxin-fused enzyme, SDS-PAGE | Cryptopygus antarcticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | Cryptopygus antarcticus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptopygus antarcticus | C1IE32 | gene CaLam | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
laminarin + H2O | - |
Cryptopygus antarcticus | ? | - |
? | |
laminarin + H2O | CaLam digests laminarin and laminarioligosaccharides except laminaribiose as an endo-beta-1,3-glucanase, releasing glucose, laminaribiose and laminaritriose as the major products | Cryptopygus antarcticus | D-glucose + laminaribiose + laminaritriose | - |
? | |
laminarioligosaccharide + H2O | CaLam digests laminarin and laminarioligosaccharides except laminaribiose as an endo-beta-1,3-glucanase, releasing glucose, laminaribiose and laminaritriose as the major products | Cryptopygus antarcticus | D-glucose + laminaribiose + laminaritriose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28983, sequence calculation, x * 46000, recombinant His-tagged and thioredoxin-fused enzyme, SDS-PAGE | Cryptopygus antarcticus |
More | the enzyme contains a putative signal peptide and a typical motif of glycosyl hydrolase family 16, EIDITE, CaLam homology modelling, overview | Cryptopygus antarcticus |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,3-glucanase | - |
Cryptopygus antarcticus |
More | the enzyme belongs to the glycosyl hydrolase family 16, GH16 | Cryptopygus antarcticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Cryptopygus antarcticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Cryptopygus antarcticus |