Literature summary for 3.2.1.39 extracted from

Takeda, T.; Nakano, Y.; Takahashi, M.; Konno, N.; Sakamoto, Y.; Arashida, R.; Marukawa, Y.; Yoshida, E.; Ishikawa, T.; Suzuki, K.
Identification and enzymatic characterization of an endo-1,3-beta-glucanase from Euglena gracilis (2015), Phytochemistry, 116, 21-27.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Aspergillus oryzae	Euglena gracilis

Inhibitors

Inhibitors	Comment	Organism	Structure
Co2+	1 mM, 40-50% inhibition	Euglena gracilis
Cu2+	1 mM, 40-50% inhibition	Euglena gracilis
Fe2+	1 mM, 40-50% inhibition	Euglena gracilis
Mn2+	1 mM, 40-50% inhibition	Euglena gracilis
additional information	not inhibitory: NaCl up to 100 mM, or 1 mM MgCl2, KCl, CaCl2 and ZnSO4	Euglena gracilis
Ni2+	1 mM, 85% inhibition	Euglena gracilis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	Laminarin	Km value 0.21 mg/ml, pH 5.0, 50°C	Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	-	Euglena gracilis

Organism

Organism	UniProt	Comment	Textmining
Euglena gracilis	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	sequence contains a putative signal peptide consisting of 16 amino acids	Euglena gracilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alkaline-treated paramylon + H2O	78.6% of the activity with laminarin	Euglena gracilis	?	-	?
laminarin + H2O	-	Euglena gracilis	D-glucose + laminaribiose + laminaritriose	-	?
additional information	enzyme additionally catalyzes a transglycosylation reaction by which reaction products with a higher molecular weight than the supplied substrates are initially generated. Ultimately the substrates are degraded into glucose, laminaribiose and laminaritriose	Euglena gracilis	?	-	?
paramylon + H2O	23.4% of the activity with laminarin	Euglena gracilis	?	the enzyme effectively produces soluble beta-1,3-glucans from alkaline-treated Euglena freeze-dried powder containing paramylon	?

Subunits

Subunits	Comment	Organism
?	x * 40000, SDS-PAGE and calculated	Euglena gracilis

Synonyms

Synonyms	Comment	Organism
Cel17A	-	Euglena gracilis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	-	Euglena gracilis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	5.5	-	Euglena gracilis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Euglena gracilis	calculated	-	4.3

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Release 2023.1 (January 2023)