Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by metal ions | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83400 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | A0A223GEG1 | - |
- |
Pseudomonas aeruginosa CAU 342A | A0A223GEG1 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
curdlan + H2O | 100% activity. When curdlan is used as the substrate, hydrolysis products mainly range from glucose to tetrasaccharide | Pseudomonas aeruginosa | ? | - |
? | |
curdlan + H2O | 100% activity. When curdlan is used as the substrate, hydrolysis products mainly range from glucose to tetrasaccharide | Pseudomonas aeruginosa CAU 342A | ? | - |
? | |
laminarin + H2O | 72.2% activity compared to curdlan. When laminarin is used as the substrate, major products are a series of beta-1,3-oligosaccharides with degree of polymerization from 1 to 5 | Pseudomonas aeruginosa | ? | - |
? | |
laminarin + H2O | 72.2% activity compared to curdlan. When laminarin is used as the substrate, major products are a series of beta-1,3-oligosaccharides with degree of polymerization from 1 to 5 | Pseudomonas aeruginosa CAU 342A | ? | - |
? | |
additional information | the enzyme does not display activity towards agarose, pachyman, Zymosan A, barley beta-glucan, and (carboxymethyl)cellulose | Pseudomonas aeruginosa | ? | - |
? | |
additional information | the enzyme does not display activity towards agarose, pachyman, Zymosan A, barley beta-glucan, and (carboxymethyl)cellulose | Pseudomonas aeruginosa CAU 342A | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 79300, calculated from amino acid sequence | Pseudomonas aeruginosa |
monomer | 1 * 86900, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
Bglu50A | - |
Pseudomonas aeruginosa |
endo-beta-1,3-D-glucanase | - |
Pseudomonas aeruginosa |
endo-type beta-1,3-glucanase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | the enzyme is almost completely inactivated at 50°C after 30 min. The half-lives of the enzyme at 40, 45, and 50°C are determined to be 872, 83 and 7 min, respectively | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | the enzyme retains more than 80% of its maximal activity after incubation at 30°C within pH 4.0-8.0 for 30 min | Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | calculated from amino acid sequence | - |
5.7 |