Literature summary for 3.2.1.39 extracted from

Ippel, J.H.; Koutsopoulos, S.; Nabuurs, S.M.; van Berkel, W.J.; van der Oost, J.; van Mierlo, C.P.
NMR characterization of a 264-residue hyperthermostable endo-beta-1,3-glucanase (2010), Biochem. Biophys. Res. Commun., 391, 370-375.

Search for more literature for 3.2.1.39

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted to the culture medium	Pyrococcus furiosus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	bound to the enzyme. Calcium binding results in relatively small chemical shift changes in a region distant from the active site of pfLamA and thus causes only minor conformational modifications. Removal of calcium does not significantly alter the denaturation temperature of pfLamA, implying that calcium does not stabilize the enzyme against global unfolding	Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30135	-	x * 30135, mass spectrometry	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	-	gene lamA	-

Purification (Commentary)

Purification (Comment)	Organism
native LamA from culture medium by hydrophobic interaction chromatography and gel filtration	Pyrococcus furiosus

Subunits

Subunits	Comment	Organism
?	x * 30135, mass spectrometry	Pyrococcus furiosus
More	LamA NMR, differential scanning calorimetry, and mass spectrometry structure determination and analysis, modelling, overview	Pyrococcus furiosus

Synonyms

Synonyms	Comment	Organism
endo-beta-1,3-glucanase	-	Pyrococcus furiosus
PfLamA	-	Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
104	-	-	Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
62	104	active at 62°C, most active at 104°C, not active at 20°C	Pyrococcus furiosus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)