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Literature summary for 3.2.1.39 extracted from
- Characterization of PCEng2 a beta-1,3-endoglucanase homologue in Pneumocystis carinii with activity in cell wall regulation (2009), Am. J. Respir. Cell Mol. Biol., 43, 192-200.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PCEng2, DNA and amino acid sequence determination and analysis, expression of recombinant His6-tagged PCEng2 in Saccharomyces cerevisiae, complementation of Eng2-deleted Saccharomyces cerevisiae strains with restoration of the cell wall separation defect during proliferation | Pneumocystis carinii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | - |
Pneumocystis carinii | 5618 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
x * 75000, recombinant His6-tagged enzyme, SDS-PAGE | Pneumocystis carinii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pneumocystis carinii | B3VTS4 | isolated from female Long Evans rats, gene PCEng2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged PCEng2 from Saccharomyces cerevisiae by immunoaffinity chromatography and nickel affinity chromatography | Pneumocystis carinii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cyst | PCEng2 mRNA expression during the life cycle of Pneumocystis carinii, upregulation in cystic isolates, the protein occurs in both cyst and trophic forms, overview. PCEng2 mRNA expression is largely restricted to cysts and is not pH-responsive | Pneumocystis carinii | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000, recombinant His6-tagged enzyme, SDS-PAGE | Pneumocystis carinii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-1,3-endoglucanase | - |
Pneumocystis carinii |
| More | the enzyme shows similarities to fungal glycosyl hydrolases GH family 81 | Pneumocystis carinii |
| PCEng2p | - |
Pneumocystis carinii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | PCEng2 plays a significant role in cell wall regulation, the glucan-rich cyst cell wall is essential for cel viability, and regulation of cyst cell wall beta-glucans is a fundamental process during completion of the Pneumocystis life cycle | Pneumocystis carinii |
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