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Literature summary for 3.2.1.39 extracted from
- Purification and characterisation of endo-beta-1,4-glucanase and laminarinase enzymes from the gecarcinid land crab Gecarcoidea natalis and the aquatic crayfish Cherax destructor (2008), J. Exp. Biol., 211, 2275-2287.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
SDS-PAGE | Gecarcoidea natalis |
| 71000 | - |
gel filtration chromatography | Gecarcoidea natalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gecarcoidea natalis | - |
- |
- |
| Gecarcoidea natalis Pocock 1888 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C | Gecarcoidea natalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| midgut | - |
Gecarcoidea natalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.096 | - |
homogenate | Gecarcoidea natalis |
| 0.252 | - |
after DEAE anion exchange chromatography | Gecarcoidea natalis |
| 0.945 | - |
after HIC chromatography | Gecarcoidea natalis |
| 7.8 | - |
after gel filtration chromatography | Gecarcoidea natalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| laminarin + H2O | - |
Gecarcoidea natalis | D-glucose + laminarioligosaccharides | - |
? |  |
| laminarin + H2O | - |
Gecarcoidea natalis Pocock 1888 | D-glucose + laminarioligosaccharides | - |
? | |
| additional information | laminarinase mainly hydrolyses beta?1,3-glycosidic bonds, with negligible activity towards beta-1,4-glycosidic bonds | Gecarcoidea natalis | ? | - |
? | |
| additional information | laminarinase mainly hydrolyses beta?1,3-glycosidic bonds, with negligible activity towards beta-1,4-glycosidic bonds | Gecarcoidea natalis Pocock 1888 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | 41000 SDS-PAGE, 71000 gel filtration chromatography, lamarinase is a dimer or contains a laminarin-binding domain, which is in similar size to the catalytic compound | Gecarcoidea natalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,3-beta-glucanase | - |
Gecarcoidea natalis |
| laminarinase | - |
Gecarcoidea natalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Gecarcoidea natalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | - |
Gecarcoidea natalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | - |
Gecarcoidea natalis |
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