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Literature summary for 3.2.1.39 extracted from

  • Abraham, A.; Narayanan, S.; Philip, S.; Nair, D.; Chandrasekharan, A.; Kochupurackal, J.
    In silico characterization of a novel beta-1,3-glucanase gene from Bacillus amyloliquefaciens - A bacterial endophyte of Hevea brasiliensis antagonistic to Phytophthora meadii (2013), J. Mol. Model., 19, 999-1007.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic tree and sequence comparisons Bacillus amyloliquefaciens

Crystallization (Commentary)

Crystallization (Comment) Organism
structure homology modelling Bacillus amyloliquefaciens

Protein Variants

Protein Variants Comment Organism
I157L site-directed mutagenesis of the active site residue Bacillus amyloliquefaciens
additional information mutant docking study, overview Bacillus amyloliquefaciens
N52D site-directed mutagenesis of the active site residue Bacillus amyloliquefaciens
V158A site-directed mutagenesis of the active site residue Bacillus amyloliquefaciens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted, it contains a cleavage site exists between amino acid positions 25 and 26 Bacillus amyloliquefaciens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27790
-
x * 27790, sequence calculation Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens
-
endophytic in Hevea brasiliensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
53 76 different isolates, pH and temperature not specified in the publication Bacillus amyloliquefaciens

Subunits

Subunits Comment Organism
? x * 27790, sequence calculation Bacillus amyloliquefaciens

Synonyms

Synonyms Comment Organism
beta-1,3-glucanase
-
Bacillus amyloliquefaciens

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus amyloliquefaciens sequence calculation
-
5.93

General Information

General Information Comment Organism
additional information phylogenetic tree, sequence comparisons and structure homology modelling, residues Asn52, Ile157 and Val158 form the active site Bacillus amyloliquefaciens