Literature summary for 3.2.1.39 extracted from

Zaslona, H.; Trusek-Holownia, A.; Radosinski, L.; Hennig, J.
Optimization and kinetic characterization of recombinant 1,3-beta-glucanase production in Escherichia coli K-12 strain BL21/pETSD10 - a bioreactor scale study (2015), Lett. Appl. Microbiol., 61, 36-43.

Search for more literature for 3.2.1.39

Application

Application	Comment	Organism
synthesis	heterologous 1,3-beta-glucanase production in Escherichia coli is favoured with moderate culture aeration (0.7-0.9 vvm) and moderate stirring (125150 rev/min)	Solanum tuberosum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36500	-	x * 36500, SDS-PAGE	Solanum tuberosum

Organism

Organism	UniProt	Comment	Textmining
Solanum tuberosum	Q70C53	cv. Desiree	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
252	-	enzyme isolated from culture with aeration at 135 rev/min and stirring at 0.8 vvm, pH 5.6, 28°C	Solanum tuberosum

Storage Stability

Storage Stability	Organism
at 28°C, pH 5.6, half-life is 202 h	Solanum tuberosum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carboxymethyl curdlan + H2O	-	Solanum tuberosum	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 36500, SDS-PAGE	Solanum tuberosum

Synonyms

Synonyms	Comment	Organism
GLUB20-2	-	Solanum tuberosum

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Release 2023.1 (January 2023)