Information on EC 1.5.1.3 - dihydrofolate reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
1.5.1.3
-
RECOMMENDED NAME
GeneOntology No.
dihydrofolate reductase
PATHWAY
KEGG Link
MetaCyc Link
Folate biosynthesis
-
folate transformations II
-
formylTHF biosynthesis
-
Metabolic pathways
-
One carbon pool by folate
-
tetrahydrofolate biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase
The enzyme from animals and some micro-organisms also slowly reduces folate to 5,6,7,8-tetrahydrofolate.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5,6,7,8-tetrahydrofolate: NADP+ oxidoreductase
-
-
7,8-dihydrofolate reductase
-
-
-
-
dehydrogenase, tetrahydrofolate
-
-
-
-
DHFR
-
-
-
-
DHFR
J7GQW2
-
DHFR
Q6FPH0
-
DHFR
Q6IQS4
-
DHFR
-
-
DHFR
O30463
-
DHFR type IIIC
-
-
-
-
DHFR-TS
B5U9U8
-
dihydrofolate reductase
-
-
dihydrofolate reductase
-
-
dihydrofolate reductase
-
-
dihydrofolate reductase
D1MX72
-
dihydrofolate reductase
D1MX71
-
dihydrofolate reductase
-
-
dihydrofolate reductase
D1MX70
-
dihydrofolate reductase
-
-
dihydrofolate reductase
D1MYR1
-
dihydrofolate reductase
-
-
dihydrofolate reductase
-
-
dihydrofolate reductase
-
-
dihydrofolate reductase
-
-
dihydrofolate reductase-thymidylate synthase
-
-
-
-
dihydrofolate reductase-thymidylate synthase
B5U9U8
-
dihydrofolate reductase:thymidylate synthase
-
-
-
-
dihydrofolic acid reductase
-
-
-
-
dihydrofolic reductase
-
-
-
-
EC 1.5.1.4
-
-
formerly
-
folic acid reductase
-
-
-
-
folic reductase
-
-
-
-
mjDHFR
D1MX72
-
myDHFR
D1MX71
-
NADPH-dihydrofolate reductase
-
-
-
-
pteridine reductase:dihydrofolate reductase
-
-
-
-
R-plasmid-encoded dihydrofolate reductase
P00383
-
R67 DHFR
P00383
-
R67 dihydrofolate reductase
P00383
-
reductase, dihydrofolate
-
-
-
-
S3DHFR
Staphylococcus aureus CM.S2
-
-
-
svDHFR
D1MYR1
-
tetrahydrofolate dehydrogenase
-
-
-
-
thymidylate synthase-dihydrofolate reductase
-
bifunctional enzyme
thymidylate synthetase-dihydrofolate reductase
-
-
-
-
Trimethoprim resistance protein
-
-
-
-
TS-DHFR
-
bifunctional enzyme
CAS REGISTRY NUMBER
COMMENTARY
9002-03-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
gene dhfr-ts
UniProt
Manually annotated by BRENDA team
expression in Escherichia coli; strain C-125
-
-
Manually annotated by BRENDA team
overview
-
-
Manually annotated by BRENDA team
2 forms
-
-
Manually annotated by BRENDA team
overview
-
-
Manually annotated by BRENDA team
trimethoprim-susceptible strain PC 174 and trimethoprim-resistant strain PC 178
-
-
Manually annotated by BRENDA team
chinese hamster, methotrexate-sensitive and methotrexate-resistant cells
-
-
Manually annotated by BRENDA team
2 forms: I, II; baby hamster kidney cells
-
-
Manually annotated by BRENDA team
baby hamster kidney cells
-
-
Manually annotated by BRENDA team
wild type and methotrexate-resistant
-
-
Manually annotated by BRENDA team
bifunctional enzyme: dihydrofolate reductase-thymidylate synthase EC 2.1.1.45
-
-
Manually annotated by BRENDA team
bifunctional enzyme: pteridine reductase-dihydrofolate reductase
-
-
Manually annotated by BRENDA team
three different forms I, IIa and IIb
-
-
Manually annotated by BRENDA team
wild carrot, enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function
-
-
Manually annotated by BRENDA team
expression in Chinese hamster ovary cells
Uniprot
Manually annotated by BRENDA team
R-plasmid encoded, trimethoprim-resistant
-
-
Manually annotated by BRENDA team
Enterococcus faecalis R
R
-
-
Manually annotated by BRENDA team
2 forms termed mutant type and wild-type enzyme in a single organism; amethopterin-resistant strain; strain A, var. durans
-
-
Manually annotated by BRENDA team
amethopterin-resistant strain
-
-
Manually annotated by BRENDA team
Enterococcus faecium A
strain A, var. durans
-
-
Manually annotated by BRENDA team
amethopterin-resistant
-
-
Manually annotated by BRENDA team
isoenzymes type I and type II, resistant to trimethoprim
-
-
Manually annotated by BRENDA team
isozyme type II, R-plasmid encoded isoform R67
UniProt
Manually annotated by BRENDA team
isozyme type II, R-plasmid encoded; trimethoprim-resistant strain
-
-
Manually annotated by BRENDA team
methotrexate-resistant mutant; strain MB 1428, B
-
-
Manually annotated by BRENDA team
plasmid-encoded isoform R67
-
-
Manually annotated by BRENDA team
plasmid-encoded isoform R67
UniProt
Manually annotated by BRENDA team
plasmid-encoded R67 isoform
UniProt
Manually annotated by BRENDA team
seleno- and telluromethionine-containing enzyme
Uniprot
Manually annotated by BRENDA team
strain B ATCC 11303; strain MB 1428, B
-
-
Manually annotated by BRENDA team
strain MB 1428, B
-
-
Manually annotated by BRENDA team
strains: MB3746, MB 3747 of K12; trimethoprim-resistant strain
-
-
Manually annotated by BRENDA team
trimethoprim-resistant strain
-
-
Manually annotated by BRENDA team
various strains
-
-
Manually annotated by BRENDA team
wild-type and mutants
-
-
Manually annotated by BRENDA team
Escherichia coli MB 1428
strain MB 1428, B
-
-
Manually annotated by BRENDA team
overview
-
-
Manually annotated by BRENDA team
extremely halophilic archaebacterium
-
-
Manually annotated by BRENDA team
extremely halophilic archaebacterium; isozyme hDHFR-1 from gene hdrA and isozyme hDHFR-2 from gene hdrB
-
-
Manually annotated by BRENDA team
i.e. Helicoverpa virescens, tobacco budworm
-
-
Manually annotated by BRENDA team
c.86 + 60_78 insertion/deletion polymorphism is associated with serum and red blood cell folate concentrations in women
-
-
Manually annotated by BRENDA team
cancer patients with and without thrombosis
-
-
Manually annotated by BRENDA team
children with acute lymphoblastic leukemia
-
-
Manually annotated by BRENDA team
cultured mammalian cells, overview
-
-
Manually annotated by BRENDA team
methotrexate-resistant WIL-2 lymphoblastoid cells
-
-
Manually annotated by BRENDA team
methotrexate-resistant WIL-2 lymphoblastoid cells; methotrexate-sensitive cell line HeLa BU-25, methotrexate-insensitive cell line VA2-B
-
-
Manually annotated by BRENDA team
mothers of a spina bifida affected child, spina biWda patients, and controls
-
-
Manually annotated by BRENDA team
patients with osteosarcoma
-
-
Manually annotated by BRENDA team
human virus produced in cultured lymphoma derived B-cells
-
-
Manually annotated by BRENDA team
amethopterin-resistant
-
-
Manually annotated by BRENDA team
amethopterin-resistant; isozymes I and II
-
-
Manually annotated by BRENDA team
amethopterin-resistant; methotrexate-resistant
-
-
Manually annotated by BRENDA team
antifolate-resistant strain
-
-
Manually annotated by BRENDA team
methotrexate-resistant
-
-
Manually annotated by BRENDA team
bifunctional enzyme dihydrofolate reductase-thymidylate synthase
-
-
Manually annotated by BRENDA team
bifunctional enzyme dihydrofolate reductase-thymidylate synthase; methotrexate-resistant strain with altered enzyme
-
-
Manually annotated by BRENDA team
methotrexate resistant bifunctional enzyme: thymidylate synthase-dihydrofolate reductase
-
-
Manually annotated by BRENDA team
cultured mammalian cells, overview
-
-
Manually annotated by BRENDA team
sp. nov.
Uniprot
Manually annotated by BRENDA team
altered enzyme encoded by amplified genes in cultured fibroblasts
-
-
Manually annotated by BRENDA team
L1210 lymphoma cells
-
-
Manually annotated by BRENDA team
L1210 lymphoma cells, amethopterin-resistant
-
-
Manually annotated by BRENDA team
multiple forms
-
-
Manually annotated by BRENDA team
overview
-
-
Manually annotated by BRENDA team
2 forms, not genetically determined isozymes
-
-
Manually annotated by BRENDA team
Pigeon
-
-
-
Manually annotated by BRENDA team
Pigeon
overview
-
-
Manually annotated by BRENDA team
bifunctional enzyme: dihydrofolate reductase-thymidylate synthase EC 2.1.1.45
-
-
Manually annotated by BRENDA team
pyrimethamine-sensitive and pyrimethamine-resistant strain
-
-
Manually annotated by BRENDA team
bifunctional thymidylate synthase-dihydrofolate reductase
Uniprot
Manually annotated by BRENDA team
isolated from infected Kenyan adults
-
-
Manually annotated by BRENDA team
isolated from Sudanese patients treated with sulphadoxine/pyrimethamine or sulphadoxine/pyrimethamine plus chloroquine
-
-
Manually annotated by BRENDA team
isolates from Car Nicobar Island, India
-
-
Manually annotated by BRENDA team
isolates from Cote d'Ivoire
-
-
Manually annotated by BRENDA team
isolates from Malawi
-
-
Manually annotated by BRENDA team
isolates from Mozambique
-
-
Manually annotated by BRENDA team
isolates from southern Mozambique
-
-
Manually annotated by BRENDA team
several Ser108 mutants
-
-
Manually annotated by BRENDA team
bifunctional dihydrofolate reductase/thymidylate synthase
-
-
Manually annotated by BRENDA team
fragment; bifunctional dihydrofolate reductase-thymidylate synthase
UniProt
Manually annotated by BRENDA team
isolates from Sri Lanka
-
-
Manually annotated by BRENDA team
polymorphisms in Indian field isolates
-
-
Manually annotated by BRENDA team
wild-type and mutant enzymes recombinant from Escherichia coli
-
-
Manually annotated by BRENDA team
human-derived
-
-
Manually annotated by BRENDA team
rat-derived
-
-
Manually annotated by BRENDA team
recombinant human-derived and rat-derived enzyme
-
-
Manually annotated by BRENDA team
protozoa
-
-
-
Manually annotated by BRENDA team
protozoa
overview
-
-
Manually annotated by BRENDA team
carcinoma Walker 256
-
-
Manually annotated by BRENDA team
phage type 179, R-plasmid enzyme, isozyme type III
-
-
Manually annotated by BRENDA team
green algae, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other
-
-
Manually annotated by BRENDA team
methicillin-sensitive and -resistant strains
-
-
Manually annotated by BRENDA team
strain CM.S2, trimethoprim-resistant enzyme
-
-
Manually annotated by BRENDA team
Staphylococcus aureus CM.S2
strain CM.S2, trimethoprim-resistant enzyme
-
-
Manually annotated by BRENDA team
methicillin-sensitive and -resistant strains
-
-
Manually annotated by BRENDA team
methicillin-sensitive and -resistant strains
-
-
Manually annotated by BRENDA team
methotrexate-resistant form
-
-
Manually annotated by BRENDA team
wild-type and mutant forms
-
-
Manually annotated by BRENDA team
overview
-
-
Manually annotated by BRENDA team
thermophilic bacterium
-
-
Manually annotated by BRENDA team
bifunctional dihydrofolate reductase-thymidylate synthase
-
-
Manually annotated by BRENDA team
bifuntional enzyme with dihydrofolate reductase and thymidylate synthase activity; trimethoprim-resistant strain
-
-
Manually annotated by BRENDA team
overview
-
-
Manually annotated by BRENDA team
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
50
-, Q70YQ6
20°C: about 65% of maximal activity, 50°C: about 70% of maximal activity
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.2
-
-
isoelectric focusing
PDB
SCOP
CATH
ORGANISM
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)
Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Enterococcus faecalis (strain ATCC 700802 / V583)
Enterococcus faecalis (strain ATCC 700802 / V583)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain UTI89 / UPEC)
Geobacillus kaustophilus (strain HTA426)
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)
Plasmodium falciparum (isolate K1 / Thailand)
Plasmodium falciparum (isolate K1 / Thailand)
Plasmodium falciparum (isolate K1 / Thailand)
Plasmodium falciparum (isolate K1 / Thailand)
Plasmodium falciparum (isolate K1 / Thailand)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Staphylococcus aureus (strain bovine RF122 / ET3-1)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
for equilibrium and kinetic folding mechanism, a continuous adenosine-binding domain is required for a stable thermal intermediate and a continuous discontinuous loop domain is required for a stable urea intermediate
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
8
-
maximal stability
6
8
-
the enzyme is most stable at pH 7.0, with a half-life for activity loss of around 13 days. Loss of activity is fastest at pH 5 with a half-life of 41 min, while the half-life of activity loss at pH 9.5 is about 2 h
9
-
-
rapid decrease in stability above