Any feedback?
Literature summary for 1.5.1.3 extracted from
- Large-scale purification and characterization of dihydrofolate reductase from a methotrexate-resistant strain of Lactobacillus casei (1976), Biochem. J., 157, 559-571.
General Stability
| General Stability | Organism |
|---|---|
| frozen solutions are not stable for long periods | Lacticaseibacillus casei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | - |
Lacticaseibacillus casei | |
| NADP+ | - |
Lacticaseibacillus casei |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Lacticaseibacillus casei |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | activates | Lacticaseibacillus casei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17900 | - |
gel filtration | Lacticaseibacillus casei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus casei | - |
methotrexate-resistant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lacticaseibacillus casei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Lacticaseibacillus casei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, freeze-dried, several months | Lacticaseibacillus casei |
| 4°C, a few days | Lacticaseibacillus casei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Lacticaseibacillus casei | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | - |
Lacticaseibacillus casei | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
folate | - |
Lacticaseibacillus casei | |
| 4 | - |
7,8-dihydrofolate | + NADPH | Lacticaseibacillus casei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Lacticaseibacillus casei |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | 8.5 | half-maximal activities at pH 5.9 and pH 8.5 | Lacticaseibacillus casei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Lacticaseibacillus casei | |
| NADPH | - |
Lacticaseibacillus casei | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Lacticaseibacillus casei | |
| 0.0018 | - |
NADP+ | - |
Lacticaseibacillus casei | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
