Any feedback?
Literature summary for 1.5.1.3 extracted from
- Development of a yeast protein fragment complementation assay (PCA) system using dihydrofolate reductase (DHFR) with specific additives (2008), Appl. Microbiol. Biotechnol., 80, 735-743.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | development of a yeast protein fragment complementation assay system using dihydrofolate reductase and application for investigating eukaryotic protein-protein interaction in vivo. Fusion of human oncoprotein Ras and Ras-binding domain of Raf-1 to dihydrofolate reductase as a model and evaluation of interaction between these proteins | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| folic acid | use in yeast protein fragment complementation assay system to prevent endogenous dihydrofolate reductase activity | Saccharomyces cerevisiae |  |
| sulfanilamide | use in yeast protein fragment complementation assay system to prevent endogenous dihydrofolate reductase activity | Saccharomyces cerevisiae | |
| trimethoprim | use in yeast protein fragment complementation assay system to prevent endogenous dihydrofolate reductase activity | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
