EC Number   |
|---|
    1.5.1.3 | - |
| 1.5.1.3 | a baculovirus system is established for the expression of this DHFR in Bombyx mori chrysalides. To isolate the expressed protein, whole infected pupae are homogenized |
| 1.5.1.3 | a dihydrofolate reductase-like sequence, DHFRLS, is encoded on chromosome 4, orfs 3139757-3140178 and 413244-415704, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis. RT-qPCR analysis shows no evidence of expression of the DHFRLS in rat |
| 1.5.1.3 | a dihydrofolate reductase-like sequence, DHFRLS, is encoded on chromosome 9, orf 31567433:31568480, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis |
| 1.5.1.3 | a variant (Xet-3) of dihydrofolate reductase from Escherichia coli constructed by introducing residues located at the Thermotoga maritima dihydrofolate reductase (TmDHFR) dimer interface is expressed in Escherichia coli BL21(DE3) cells |
| 1.5.1.3 | amplification and DNA-sequencing of full length gene from DNA-library |
| 1.5.1.3 | cloning of gene encoding mutant R53M enzyme from methotrexate-resistant strain into an expression plasmid, expression in Escherichia coli |
| 1.5.1.3 | DNA-sequence analysis |
| 1.5.1.3 | expresion in Escherichia coli and CHO cell |
| 1.5.1.3 | expressed in Escherichia coli |
