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Literature summary for 1.5.1.3 extracted from
- Substrate specificity analysis of dihydrofolate/dihydromethanopterin reductase homologs in methylotrophic alpha-proteobacteria (2018), Front. Microbiol., 9, 2439 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
7,8-dihydrofolate | pH 6.8, 23°C | Methylorubrum extorquens |  |
| 0.018 | - |
7,8-dihydrofolate | pH 6.8, 23°C | Methylorubrum extorquens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylorubrum extorquens | Q8KQF2 | isoform DfrB | - |
| Methylorubrum extorquens | Q8KQF3 | isoform DFRa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.011 | - |
substrate dihydrosarcinapterin, pH 6.8, 23°C | Methylorubrum extorquens |
| 0.041 | - |
substrate dihydrosarcinapterin, pH 6.8, 23°C | Methylorubrum extorquens |
| 3.13 | - |
substrate 7,8-dihydrofolate, pH 6.8, 23°C | Methylorubrum extorquens |
| 18.5 | - |
substrate 7,8-dihydrofolate, pH 6.8, 23°C | Methylorubrum extorquens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH + H+ | - |
Methylorubrum extorquens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| dihydrosarcinapterin + NADPH + H+ | - |
Methylorubrum extorquens | tetrahydrosarcinapterin + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DfrA | - |
Methylorubrum extorquens |
| DfrB | - |
Methylorubrum extorquens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 37 | broad | Methylorubrum extorquens |
| 23 | 40 | broad | Methylorubrum extorquens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.2 | - |
7,8-dihydrofolate | pH 6.8, 23°C | Methylorubrum extorquens | |
| 17 | - |
7,8-dihydrofolate | pH 6.8, 23°C | Methylorubrum extorquens | |
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Release 2023.1 (January 2023)
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