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Literature summary for 1.5.1.3 extracted from

  • Haenggi, U.J.; Littlefield, J.W.
    Isolation and characterization of the multiple forms of dihydrofolate reductase from methotrexate-resistant hamster cells (1974), J. Biol. Chem., 249, 1390-1397.
    View publication on PubMed
Search for more literature for 1.5.1.3

Activating Compound

Activating Compound Comment Organism Structure
Urea activates at 1 M Cricetulus sp.

General Stability

General Stability Organism
bovine serum albumin protects against inactivation Cricetulus sp.
unusually resistant to trypsin Cricetulus sp.

Inhibitors

Inhibitors Comment Organism Structure
methotrexate
-
 Cricetulus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulation Cricetulus sp.

Organism

Organism UniProt Comment Textmining
Cricetulus sp.
-
 2 forms: I, II
-
Cricetulus sp.
-
 baby hamster kidney cells
-

Purification (Commentary)

Purification (Comment) Organism
baby hamster kidney cells, 2 forms: I, II Cricetulus sp.

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Cricetulus sp.
-

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 inactivation constant: 0.14 per min form I, 0.13 per min form II Cricetulus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
 R-plasmid encoded enzyme, 2 optima, pH 4.2 and pH 7.8, in absence of KCl Cricetulus sp.
4.8
-
 in presence of KCl Cricetulus sp.

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH-activity profile in absence and presence of KCl Cricetulus sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Cricetulus sp.