EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
1.5.1.3 | -999 |
- |
more |
- |
392252 |
1.5.1.3 | -999 |
- |
more |
analysis of kinetic and thermodynamic fitting parameters and relaxation dispersion curves at different pH values and temperatures |
685285 |
1.5.1.3 | -999 |
- |
more |
kcat for wild-type is pH-dependent, maximal kcat at pH 7.0 |
711226 |
1.5.1.3 | -999 |
- |
more |
pyrimethamine sensitive and resistant strain |
392247 |
1.5.1.3 | -999 |
- |
more |
selenomethionine-containing mutants |
392286 |
1.5.1.3 | 0.003 |
- |
7,8-dihydrofolate |
pH 7, 20°C, mutant enzyme V11D |
747067 |
1.5.1.3 | 0.02 |
- |
folate |
- |
392242 |
1.5.1.3 | 0.02 |
- |
NADPH |
pH 7.0, 30°C, mutant D31L |
686658 |
1.5.1.3 | 0.025 |
- |
7,8-dihydrofolate |
pH 7.0, 30°C, mutant D31L |
686658 |
1.5.1.3 | 0.05 |
- |
7,8-dihydrofolate |
pH 7, 20°C, dimeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface |
747067 |