Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium avium |
Protein Variants | Comment | Organism |
---|---|---|
D31A | 7% of wild-type activity | Mycobacterium avium |
D31E | 18.9% of wild-type activity | Mycobacterium avium |
D31L | 0.39% of wild-type activity | Mycobacterium avium |
D31N | 15.4% of wild-type activity | Mycobacterium avium |
D31Q | 14.7% of wild-type activity | Mycobacterium avium |
L32A | 96% of wild-type activity. 12-fold decrease in affinity for trimethoprim | Mycobacterium avium |
L32D | 33% of wild-type activity. 7-fold decrease in affinity for trimethoprim | Mycobacterium avium |
L32F | 103% of wild-type activity | Mycobacterium avium |
V76A | 98.7% of wild-type activity | Mycobacterium avium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
trimethoprim | - |
Mycobacterium avium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00065 | - |
NADPH | pH 7.0, 30°C, mutant D31Q | Mycobacterium avium | |
0.00068 | - |
NADPH | pH 7.0, 30°C, mutant D31A | Mycobacterium avium | |
0.00068 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant L32F | Mycobacterium avium | |
0.0007 | - |
7,8-dihydrofolate | pH 7.0, 30°C, wild-type | Mycobacterium avium | |
0.00077 | - |
NADPH | pH 7.0, 30°C, mutant D31N | Mycobacterium avium | |
0.00078 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant V76A | Mycobacterium avium | |
0.00084 | - |
NADPH | pH 7.0, 30°C, mutant L32D | Mycobacterium avium | |
0.00096 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant L32A | Mycobacterium avium | |
0.0013 | - |
NADPH | pH 7.0, 30°C, mutant L32F | Mycobacterium avium | |
0.0014 | - |
NADPH | pH 7.0, 30°C, mutant D31L | Mycobacterium avium | |
0.0014 | - |
NADPH | pH 7.0, 30°C, mutant L32A | Mycobacterium avium | |
0.0015 | - |
NADPH | pH 7.0, 30°C, mutant V76A | Mycobacterium avium | |
0.0015 | - |
NADPH | pH 7.0, 30°C, wild-type | Mycobacterium avium | |
0.0019 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant D31E | Mycobacterium avium | |
0.002 | - |
NADPH | pH 7.0, 30°C, mutant D31E | Mycobacterium avium | |
0.0021 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant D31N | Mycobacterium avium | |
0.0023 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant D31Q | Mycobacterium avium | |
0.0051 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant L32D | Mycobacterium avium | |
0.037 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant D31A | Mycobacterium avium | |
0.198 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant D31L | Mycobacterium avium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium avium | O30463 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Mycobacterium avium | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADPH | pH 7.0, 30°C, mutant D31L | Mycobacterium avium | |
0.025 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant D31L | Mycobacterium avium | |
0.59 | - |
NADPH | pH 7.0, 30°C, mutant D31A | Mycobacterium avium | |
0.86 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant D31A | Mycobacterium avium | |
1.5 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant D31Q | Mycobacterium avium | |
2 | 3.7 | NADPH | pH 7.0, 30°C, mutant V76A | Mycobacterium avium | |
2.05 | - |
NADPH | pH 7.0, 30°C, mutant D31E | Mycobacterium avium | |
2.18 | - |
NADPH | pH 7.0, 30°C, mutant D31Q | Mycobacterium avium | |
3.58 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant D31E | Mycobacterium avium | |
5.03 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant D31N | Mycobacterium avium | |
7.2 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant L32D | Mycobacterium avium | |
8.5 | - |
NADPH | pH 7.0, 30°C, mutant D31N | Mycobacterium avium | |
10 | - |
NADPH | pH 7.0, 30°C, mutant L32D | Mycobacterium avium | |
16.2 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant L32A | Mycobacterium avium | |
17.8 | - |
NADPH | pH 7.0, 30°C, mutant L32A | Mycobacterium avium | |
18.7 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant V76A | Mycobacterium avium | |
20.5 | - |
7,8-dihydrofolate | pH 7.0, 30°C, wild-type | Mycobacterium avium | |
21 | - |
7,8-dihydrofolate | pH 7.0, 30°C, mutant L32F | Mycobacterium avium | |
23.2 | - |
NADPH | pH 7.0, 30°C, mutant L32F | Mycobacterium avium | |
26.8 | - |
NADPH | pH 7.0, 30°C, wild-type | Mycobacterium avium |