Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Moritella profunda |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | Moritella profunda | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moritella profunda | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiPrep 16/10 SP-XL column chromatography | Moritella profunda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Moritella profunda | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHFR | - |
Moritella profunda |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37.5 | - |
the melting temperature of the apo-enzyme is 37.5°C and is not affected by pH over the range 5.0-9.0 | Moritella profunda |
40 | 50 | the enzyme is able to perform its full catalytic rate for at least 1 h at temperatures up to 40°C. At higher temperatures the steady-state rate decreases rapidly with a half-life of inactivation of around 13 min at 45°C and 7 min at 50°C | Moritella profunda |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
7,8-dihydrofolate | at pH 7.0 and 5°C | Moritella profunda | |
8.2 | - |
7,8-dihydrofolate | at pH 7.0 and 10°C | Moritella profunda | |
10.8 | - |
7,8-dihydrofolate | at pH 7.0 and 15°C | Moritella profunda | |
14.8 | - |
7,8-dihydrofolate | at pH 7.0 and 20°C | Moritella profunda | |
18.7 | - |
7,8-dihydrofolate | at pH 7.0 and 25°C | Moritella profunda | |
25.3 | - |
7,8-dihydrofolate | at pH 7.0 and 30°C | Moritella profunda | |
28.8 | - |
7,8-dihydrofolate | at pH 7.0 and 45°C | Moritella profunda | |
32.5 | - |
7,8-dihydrofolate | at pH 7.0 and 35°C | Moritella profunda | |
39.6 | - |
7,8-dihydrofolate | at pH 7.0 and 40°C | Moritella profunda |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | the enzyme is most stable at pH 7.0, with a half-life for activity loss of around 13 days. Loss of activity is fastest at pH 5 with a half-life of 41 min, while the half-life of activity loss at pH 9.5 is about 2 h | Moritella profunda |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Moritella profunda |