General Stability | Organism |
---|---|
bovine serum albumin, 1 mg/ml, stabilizes during storage of purified enzyme preparation at 5°C | Crithidia fasciculata |
bovine serum albumin, 1 mg/ml, stabilizes during storage of purified enzyme preparation at 5°C | Plasmodium berghei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56700 | - |
2 * 56700, bifunctional enzyme: thymidylate synthase-dihydrofolate reductase, SDS-PAGE | Crithidia fasciculata |
107000 | - |
gel filtration | Crithidia fasciculata |
107000 | - |
bifunctional enzyme: thymidylate synthase-dihydrofolate reductase | Crithidia fasciculata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | - |
bifunctional enzyme: dihydrofolate reductase-thymidylate synthase EC 2.1.1.45 | - |
Plasmodium berghei | - |
bifunctional enzyme: dihydrofolate reductase-thymidylate synthase EC 2.1.1.45 | - |
Purification (Comment) | Organism |
---|---|
dihydrofolate reductase-thymidylate synthase EC 2.1.1.45 | Crithidia fasciculata |
methotrexate-affinity chromatography | Crithidia fasciculata |
methotrexate-affinity chromatography | Plasmodium berghei |
Storage Stability | Organism |
---|---|
5°C, 50 mM Tris-HCl, pH 8.3, 1 mM EDTA, 1 mM dithiothreitol, 1 M KCl, 0.6 mM 7,8-dihydrofolate, 50% loss of activity overnight | Plasmodium berghei |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 56700, bifunctional enzyme: thymidylate synthase-dihydrofolate reductase, SDS-PAGE | Crithidia fasciculata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Crithidia fasciculata |