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Literature summary for 1.5.1.3 extracted from

  • Miyashita, Y.; Ohmae, E.; Ikura, T.; Nakasone, K.; Katayanagi, K.
    Halophilic mechanism of the enzymatic function of a moderately halophilic dihydrofolate reductase from Haloarcula japonica strain TR-1 (2017), Extremophiles, 21, 591-602 .
    View publication on PubMed
Search for more literature for 1.5.1.3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 7,8-dihydrofolate pH 6.0, 25°C Haloarcula japonica
0.0027
-
 NADPH pH 6.0, 25°C, presence of 200 mM NaCl Haloarcula japonica
0.0036
-
 NADPH pH 8.0, 25°C, presence of 500 mM NaCl Haloarcula japonica
0.0051
-
 7,8-dihydrofolate pH 6.0, 25°C, presence of 200 mM NaCl Haloarcula japonica
0.0053
-
 NADPH pH 8.0, 25°C, presence of 200 mM NaCl Haloarcula japonica
0.0057
-
 NADPH pH 8.0, 25°C, presence of 1000 mM NaCl Haloarcula japonica
0.0064
-
 NADPH pH 8.0, 25°C Haloarcula japonica
0.0066
-
 NADPH pH 6.0, 25°C Haloarcula japonica
0.008
-
 7,8-dihydrofolate pH 8.0, 25°C, presence of 500 mM NaCl Haloarcula japonica
0.0081
-
 NADPH pH 6.0, 25°C, presence of 500 mM NaCl Haloarcula japonica
0.0081
-
 7,8-dihydrofolate pH 6.0, 25°C, presence of 500 mM NaCl Haloarcula japonica
0.0085
-
 7,8-dihydrofolate pH 8.0, 25°C Haloarcula japonica
0.0089
-
 7,8-dihydrofolate pH 8.0, 25°C, presence of 200 mM NaCl Haloarcula japonica

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl enzyme activity increases eightfold by the addition of 500 mM NaCl at pH 6.0, fourfold by 250 mM at pH 8.0, and becomes independent of salt concentration at pH 10.0 Haloarcula japonica

Organism

Organism UniProt Comment Textmining
Haloarcula japonica
-
-
-
Haloarcula japonica TR-1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH + H+
-
 Haloarcula japonica 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
7,8-dihydrofolate + NADPH + H+
-
 Haloarcula japonica TR-1 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 NADPH pH 8.0, 25°C Haloarcula japonica
1.5
-
 7,8-dihydrofolate pH 8.0, 25°C Haloarcula japonica
2
-
 NADPH pH 8.0, 25°C, presence of 1000 mM NaCl Haloarcula japonica
2.5
-
 NADPH pH 6.0, 25°C Haloarcula japonica
2.7
-
 7,8-dihydrofolate pH 8.0, 25°C, presence of 500 mM NaCl Haloarcula japonica
2.8
-
 NADPH pH 8.0, 25°C, presence of 500 mM NaCl Haloarcula japonica
3.1
-
 7,8-dihydrofolate pH 8.0, 25°C, presence of 200 mM NaCl Haloarcula japonica
3.2
-
 NADPH pH 8.0, 25°C, presence of 200 mM NaCl Haloarcula japonica
3.4
-
 7,8-dihydrofolate pH 6.0, 25°C Haloarcula japonica
8.5
-
 NADPH pH 6.0, 25°C, presence of 200 mM NaCl Haloarcula japonica
11.7
-
 7,8-dihydrofolate pH 6.0, 25°C, presence of 200 mM NaCl Haloarcula japonica
20.3
-
 NADPH pH 6.0, 25°C, presence of 500 mM NaCl Haloarcula japonica
20.7
-
 7,8-dihydrofolate pH 6.0, 25°C, presence of 500 mM NaCl Haloarcula japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2 8 activity is almost constant Haloarcula japonica

General Information

General Information Comment Organism
metabolism the rate of hydride transfer is the rate-determining step at the basic pH region, and is independent of salt concentration. The amplitude of the DHF-binding reaction increases and the tetrahydrofolate releasing rate decreases with increasing NaCl concentration Haloarcula japonica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
200
-
 NADPH pH 8.0, 25°C Haloarcula japonica
200
-
 7,8-dihydrofolate pH 8.0, 25°C Haloarcula japonica
300
-
 7,8-dihydrofolate pH 8.0, 25°C, presence of 500 mM NaCl Haloarcula japonica
400
-
 NADPH pH 6.0, 25°C Haloarcula japonica
400
-
 NADPH pH 8.0, 25°C, presence of 1000 mM NaCl Haloarcula japonica
400
-
 7,8-dihydrofolate pH 8.0, 25°C, presence of 200 mM NaCl Haloarcula japonica
600
-
 NADPH pH 8.0, 25°C, presence of 200 mM NaCl Haloarcula japonica
800
-
 NADPH pH 8.0, 25°C, presence of 500 mM NaCl Haloarcula japonica
1600
-
 7,8-dihydrofolate pH 6.0, 25°C Haloarcula japonica
2300
-
 7,8-dihydrofolate pH 6.0, 25°C, presence of 200 mM NaCl Haloarcula japonica
2500
-
 NADPH pH 6.0, 25°C, presence of 500 mM NaCl Haloarcula japonica
2500
-
 7,8-dihydrofolate pH 6.0, 25°C, presence of 500 mM NaCl Haloarcula japonica
3200
-
 NADPH pH 6.0, 25°C, presence of 200 mM NaCl Haloarcula japonica