Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli including 13 amino acid loop | Brugia malayi |
expression in Escherichia coli including 13 amino acid loop | Wuchereria bancrofti |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(-)-epicatechin | - |
Brugia malayi | |
(-)-epicatechin | - |
Wuchereria bancrofti | |
(-)-epicatechin gallate | - |
Brugia malayi | |
(-)-epicatechin gallate | - |
Wuchereria bancrofti | |
aminopterin | - |
Brugia malayi | |
aminopterin | - |
Wuchereria bancrofti | |
methotrexate | - |
Brugia malayi | |
methotrexate | - |
Wuchereria bancrofti | |
pyrimethamine | - |
Brugia malayi | |
pyrimethamine | - |
Wuchereria bancrofti | |
raltitrexed | - |
Brugia malayi | |
raltitrexed | - |
Wuchereria bancrofti | |
trimethoprim | - |
Brugia malayi | |
trimethoprim | - |
Wuchereria bancrofti | |
vitexin | - |
Brugia malayi | |
vitexin | - |
Wuchereria bancrofti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
7,8-dihydrofolate | pH 6, 25°C | Wuchereria bancrofti | |
0.0147 | - |
7,8-dihydrofolate | pH 6, 25°C | Brugia malayi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brugia malayi | - |
- |
- |
Wuchereria bancrofti | J9F199 | sequence lacks a crucial, conserved 13 amino acid loop | - |
Purification (Comment) | Organism |
---|---|
recombinant protein, using Ni-NTA resin and imidazole concentration in the wash buffer is changed from 25 mM to 100 mM | Brugia malayi |
recombinant protein, using Ni-NTA resin and imidazole concentration in the wash buffer is changed from 25 mM to 100 mM | Wuchereria bancrofti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Brugia malayi | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH + H+ | - |
Wuchereria bancrofti | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
WUBG_00817 | - |
Wuchereria bancrofti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
7,8-dihydrofolate | pH 6, 25°C | Brugia malayi | |
7.4 | - |
7,8-dihydrofolate | pH 6, 25°C | Wuchereria bancrofti |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000005 | - |
methotrexate | pH 6, 25°C | Brugia malayi | |
0.0000007 | - |
methotrexate | pH 6, 25°C | Wuchereria bancrofti | |
0.0000017 | - |
aminopterin | pH 6, 25°C | Brugia malayi | |
0.0000021 | - |
aminopterin | pH 6, 25°C | Wuchereria bancrofti | |
0.0016 | - |
raltitrexed | pH 6, 25°C | Brugia malayi | |
0.002 | - |
raltitrexed | pH 6, 25°C | Wuchereria bancrofti | |
0.0036 | - |
pyrimethamine | pH 6, 25°C | Brugia malayi | |
0.00598 | - |
trimethoprim | pH 6, 25°C | Wuchereria bancrofti | |
0.015 | - |
trimethoprim | pH 6, 25°C | Brugia malayi | |
0.015 | - |
pyrimethamine | pH 6, 25°C | Wuchereria bancrofti |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
value above 1 mM, pH 6, 25°C | Brugia malayi | (-)-epicatechin gallate | |
additional information | - |
value above 1 mM, pH 6, 25°C | Wuchereria bancrofti | (-)-epicatechin gallate | |
additional information | - |
value above 2.5 mM, pH 6, 25°C | Brugia malayi | (-)-epicatechin | |
additional information | - |
value above 2.5 mM, pH 6, 25°C | Wuchereria bancrofti | (-)-epicatechin | |
0.0000022 | - |
pH 6, 25°C | Brugia malayi | methotrexate | |
0.0000075 | - |
pH 6, 25°C | Brugia malayi | aminopterin | |
0.000014 | - |
pH 6, 25°C | Wuchereria bancrofti | aminopterin | |
0.000018 | - |
pH 6, 25°C | Wuchereria bancrofti | methotrexate | |
0.0073 | - |
pH 6, 25°C | Brugia malayi | raltitrexed | |
0.016 | - |
pH 6, 25°C | Brugia malayi | pyrimethamine | |
0.018 | - |
pH 6, 25°C | Wuchereria bancrofti | raltitrexed | |
0.065 | - |
pH 6, 25°C | Brugia malayi | trimethoprim | |
0.083 | - |
pH 6, 25°C | Wuchereria bancrofti | trimethoprim | |
0.24 | - |
pH 6, 25°C | Brugia malayi | vitexin | |
0.24 | - |
pH 6, 25°C | Wuchereria bancrofti | vitexin | |
0.454 | - |
pH 6, 25°C | Wuchereria bancrofti | pyrimethamine |