Literature summary for 1.5.1.3 extracted from

Cody, V.; Pace, J.; Lin, L.; Gangjee, A.
The Z isomer of 2,4-diaminofuro[2,3-d]pyrimidine antifolate promotes unusual crystal packing in a human dihydrofolate reductase ternary complex (2009), Acta Crystallogr. Sect. F, 65, 762-766.

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in ternary complex with NADPH and inhibitor (Z)-2,4-diamino-5-[2-(2'-methoxyphenyl)-propenyl]-furo[2,3-d]pyrimidine, protein is incubated with a 10:1 molar excess of NADPH and the inhibitor for one h over ice prior to crystallization using the hanging drop vapor diffusion method, 0.008 ml of protein solution containing 100 mM K2HPO4, pH 6.9, and 30% saturated ammonium sulfate, over a reservoir solution of 100 mM K2HPO4, pH 6.9, and 60% saturated ammonium sulfate with 3% v/v ethanol, several days, X-ray diffraction structure determination and analysis at 1.4-1.7 A resolution	Homo sapiens

Crystallization (Comment)

Organism

enzyme in ternary complex with NADPH and inhibitor (Z)-2,4-diamino-5-[2-(2'-methoxyphenyl)-propenyl]-furo[2,3-d]pyrimidine, protein is incubated with a 10:1 molar excess of NADPH and the inhibitor for one h over ice prior to crystallization using the hanging drop vapor diffusion method, 0.008 ml of protein solution containing 100 mM K2HPO4, pH 6.9, and 30% saturated ammonium sulfate, over a reservoir solution of 100 mM K2HPO4, pH 6.9, and 60% saturated ammonium sulfate with 3% v/v ethanol, several days, X-ray diffraction structure determination and analysis at 1.4-1.7 A resolution

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P00374	-	-

Synonyms

Synonyms	Comment	Organism
DHFR	-	Homo sapiens

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Release 2023.1 (January 2023)