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Literature summary for 1.5.1.3 extracted from
- Coordinated effects of distal mutations on environmentally coupled tunneling in dihydrofolate reductase (2006), Proc. Natl. Acad. Sci. USA, 103, 15753-15758.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G121V | kinetic isotope effect study, environmentally coupled tunnelling similar to wild-type | Escherichia coli |
| M42W | kinetic isotope effect study, environmentally coupled tunnelling similar to wild-type | Escherichia coli |
| M42W/G121V | kinetic isotope effect study, major change in the nature of H transfer, leading to poor reorganization and substantial gating | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | kinetic isotope effect analysis, enzyme requires no no donor-acceptor distance fluctuations and thus no gating | Escherichia coli |
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