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Literature summary for 1.5.1.3 extracted from
- The purification of dihydrofolate reductase from Drosophila melanogaster (1990), Biochim. Biophys. Acta, 1039, 261-268.
General Stability
| General Stability | Organism |
|---|---|
| stabilized by 0.1 mg/ml bovine serum albumin and 1 mM dithiothreitol at 4°C | Drosophila melanogaster |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Drosophila melanogaster | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
gel filtration | Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Drosophila melanogaster |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
purified enzyme | Drosophila melanogaster |
Storage Stability
| Storage Stability | Organism |
|---|---|
| on ice, loss of 20-60% activity after 2 h | Drosophila melanogaster |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 17000-22000 | Drosophila melanogaster |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.7 | - |
2 optima: pH 4.7 and pH 8.5 | Drosophila melanogaster |
| 8.5 | - |
2 optima: pH 4.7 and pH 8.5 | Drosophila melanogaster |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9 | - |
Drosophila melanogaster |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Drosophila melanogaster |  |
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