Literature summary for 1.5.1.3 extracted from

Chopra, S.; Dooling, R.M.; Horner, C.G.; Howell, E.E.
A balancing act between net uptake of water during dihydrofolate binding and net release of water upon NADPH binding in R67 dihydrofolate reductase (2008), J. Biol. Chem., 283, 4690-4698.

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Protein Variants

Protein Variants	Comment	Organism
I68M	compared to wild-type, low kcat/Km (DHF) values. Mutant allows growth in presence of sorbitol up to 1.44 osmol conditions	Escherichia coli
Q67H	mutant with reasonable catalytic efficiency, but displays substrate and cofactor inhibition. Contrary to wild-type which allows growth on all sorbitol conditions until the osmolyte concentration becomes too high or cell water content becomes too low, mutant Q67H allows growth up to 1.81 osmol conditions	Escherichia coli
Y69L	compared to wild-type, low kcat/Km (DHF) values. Mutant allows growth in presence of sorbitol up to 0.81 osmol conditions	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P00383	plasmid-encoded isoform R67	-

Reaction

Reaction	Comment	Organism	Reaction ID
5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+	binding of NADPH is accompanied by release of 38 water molecules, while binding of dihydrofolate is accompanied by the net uptake of water	Escherichia coli	a

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Release 2023.1 (January 2023)