3.5.1.23: ceramidase
This is an abbreviated version!
For detailed information about ceramidase, go to the full flat file.
Word Map on EC 3.5.1.23
-
3.5.1.23
-
adipor1
-
sphingolipids
-
obesity
-
adipokine
-
agonist
-
sphingomyelinase
-
sphingomyelin
-
lysosomal
-
farber
-
obese
-
leptin
-
adipocytes
-
amp-activated
-
sphingosine-1-phosphate
-
peroxisome
-
cardiovascular
-
proliferator-activated
-
triglyceride
-
globular
-
insulin-sensitizing
-
high-fat
-
tnf
-
smases
-
adipocytokine
-
adipocyte-derived
-
monophosphate-activated
-
anti-atherogenic
-
t-cadherin
-
steatosis
-
adiposity
-
glucosylceramide
-
glycosphingolipids
-
obesity-related
-
antidiabetic
-
1-phosphate
-
seven-transmembrane
-
gaucher
-
dihydrosphingosine
-
p-ampk
-
palmitoyltransferase
-
homa-ir
-
sphingoid
-
hoarseness
-
ceramide-induced
-
sphinganine
-
ceramide-mediated
-
dihydroceramide
-
myoclonic
-
adipocyte-secreted
-
analysis
-
pharmacology
-
drug development
-
medicine
-
hypoadiponectinemia
- 3.5.1.23
-
adipor1
- sphingolipids
- obesity
-
adipokine
- agonist
- sphingomyelinase
- sphingomyelin
- lysosomal
- farber
-
obese
- leptin
- adipocytes
-
amp-activated
- sphingosine-1-phosphate
- peroxisome
- cardiovascular
-
proliferator-activated
- triglyceride
-
globular
-
insulin-sensitizing
-
high-fat
- tnf
- smases
-
adipocytokine
-
adipocyte-derived
-
monophosphate-activated
-
anti-atherogenic
-
t-cadherin
- steatosis
-
adiposity
- glucosylceramide
- glycosphingolipids
-
obesity-related
-
antidiabetic
- 1-phosphate
-
seven-transmembrane
- gaucher
- dihydrosphingosine
-
p-ampk
- palmitoyltransferase
-
homa-ir
-
sphingoid
- hoarseness
-
ceramide-induced
- sphinganine
-
ceramide-mediated
- dihydroceramide
-
myoclonic
-
adipocyte-secreted
- analysis
- pharmacology
- drug development
- medicine
-
hypoadiponectinemia
Reaction
Synonyms
(dihydro)ceramidase, AC, aCDase, ACER, ACER type 3, ACER-2, ACER1, ACER2, ACER3, acid ceramidase, acylsphingosine deacylase, adiponectin receptor, ADIPOR2, alkaline ceramidase, alkaline ceramidase 1, alkaline ceramidase 2, alkaline ceramidase 3, alkCDase, alkCDase-1, alkCDase-2, ASAH-1, ASAH1, asah1b, ASAH2, ASAH2B, ASAH3, AsahA, AtACER, AtNCER1, bwa, CBCDase, CDase, CDase II, CER2, ceramidase, Dacer, Drosophila alkaline ceramidase, glycosphingolipid ceramide deacylase, Golgi alkaline ceramidase, haCER1, intramembrane ceramidase, LsnCer, maCER1, mt-CDase, N-acylsphingosine amidohydrolase, N-acylsphingosine amidohydrolase 2, N-acylsphingosine amidohydrolase-1, N-acylsphingosine aminohydrolase, N-acylsphingosine deacylase, N-CDase, nCDase, ncer1, neutral CDase, neutral ceramidase, neutral ceramidase 2, NlnCDase, OsCDase, PaCD, PaCDase, pan-ceramidase, PHP32, phytoalkaline ceramidase, phytoCDase, Putative 32 KDA heart protein, SCDase, sphingolipid ceramide N-deacylase, Tncer, TOD1, turgor regulation defect 1, Ydc1p, yeast dihydroceramidase 1, yeast phyto-ceramidase 1, Ypc1p, znCD
ECTree
3.5.1.23 ceramidaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.5.1.23 - ceramidase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(2R,3Z)-2-([(2E)-1-hydroxy-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodec-2-en-1-yl]amino)heptadec-3-ene-1,3-diol + H2O
?
9.9% hydrolysis
-
-
ir
(4E,2S,3R)-2-N-(10-pyrenedecanoyl)-1,3,17-trihydroxy-17-(3,5-dinitrobenzoyl)-4-heptadecene + H2O
?
-
-
fluorescent product formed upon hydrolysis of the N-acyl bond
-
?
11-eicosenoyl-dihydroceramide + H2O
dihydrosphingosine + 11-eicosenoic acid
-
-
-
-
?
11-eicosenoyl-phytoceramide + H2O
phytosphingosine + 11-eicosenoic acid
-
-
-
-
?
11-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-N-[(2S,3R,4E)-1,3-dihydroxy-7-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hept-4-en-2-yl]undecanamide + H2O
?
4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O
4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide
4-nitrobenzo-2-oxa-1,3-diazoyl-N-dodecanoylsphingosine + H2O
4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid + sphingosine
C12-NBD-Cer + H2O
NBD-dodecanoic acid + D-erythro-sphingosine
-
the enzyme catalyzes also the reverse reaction of ceramide synthesis
-
-
r
C18-ceramide + H2O
stearate + sphingosine
preferred substrate, fluorescent C18 ceramide in detergent micelles
-
-
?
D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O
D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide
D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-phytoceramide + H2O
D-erythro-sphingosine + ?
-
substrate activity assay
-
-
?
D-erythro-C12-NBD-ceramide + H2O
D-erythro-sphingosine + 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid
D-erythro-C24:1-ceramide + H2O
C24:1 fatty acid + D-erythro-sphingosine
-
-
-
-
?
D-erythro-dihydrosphingosine + fatty acid
?
-
very low ceramide synthesis activity
-
-
?
D-erythro-dodecanoyl-7-nitrobenz-2-oxa-1,3-diazol-4-yl-ceramide + H2O
12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoic acid + sphingosine
D-erythro-octadecanoyl-ceramide + H2O
D-erythro-sphingosine + palmitic acid
-
mtCDase specifically hydrolyzes the D-erythro-isomer of ceramide, requirements for ceramide-enzyme interaction, ligand interaction with the enzyme occurs in a high affinity low specificity manner, in contrast to catalysis which is highly specific for D-erythro-ceramide but occurs with a lower affinity
-
-
?
D-erythro-sphingosine + (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexa-4,7,10,13,16,19-enoic acid
N-((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexa-4,7,10,13,16,19-enoyl)-D-erythro-sphingosine + H2O
-
50% of activity
-
-
r
D-erythro-sphingosine + arachidonic acid
N-arachidonyl-D-erythro-sphingosine + H2O
-
61% of activity
-
-
r
D-erythro-sphingosine + behenic acid
N-behenoyl-D-erythro-sphingosine + H2O
-
24% of activity
-
-
r
D-erythro-sphingosine + cerotic acid
N-cerotoyl-D-erythro-sphingosine + H2O
-
4% of activity
-
-
r
D-erythro-sphingosine + eicosapentaenoic acid
N-eicosapentaenyl-D-erythro-sphingosine + H2O
-
52% of activity
-
-
r
D-erythro-sphingosine + fatty acid
?
-
highest ceramide synthesis activity with D-erythro-sphingosine
-
-
?
D-erythro-sphingosine + fatty acid
N-acyl-D-erythro-sphingosine + H2O
-
-
-
-
r
D-erythro-sphingosine + lauric acid
N-lauroyl-D-erythro-sphingosine + H2O
-
72% of activity
-
-
r
D-erythro-sphingosine + lignoceric acid
N-lignoceroyl-D-erythro-sphingosine + H2O
-
6% of activity
-
-
r
D-erythro-sphingosine + linoleic acid
N-linoleoyl-D-erythro-sphingosine + H2O
-
66% of activity
-
-
r
D-erythro-sphingosine + myristic acid
N-myristoyl-D-erythro-sphingosine + H2O
-
best substrate
-
-
r
D-erythro-sphingosine + oleic acid
N-oleoyl-D-erythro-sphingosine + H2O
-
39% of activity
-
-
r
D-erythro-sphingosine + palmitic acid
N-palmitoyl-D-erythro-sphingosine + H2O
-
70% of activity, palmitic acid can not be replaced with palmityl-CoA
-
-
r
D-erythro-sphingosine + stearic acid
N-stearoyl-D-erythro-sphingosine + H2O
-
49% of activity
-
-
r
D-erythro-tetracosanoyl-sphingosine + H2O
tetracosanoate + sphingosine
i.e. D-e-C24:0-ceramide
-
-
?
dihydro-N-myristoyl-D-erythro-sphingosine + H2O
dihydro-D-erythro-sphingosine + myristate
-
55.9% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine
-
-
?
dihydro-N-palmitoyl-D-erythro-sphingosine + H2O
dihydro-D-erythro-sphingosine + palmitate
-
4.5% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine
-
-
?
ganglioside + H2O
lyso-ganglioside + fatty acid
-
GM1, GM2, GM3
corresponding to ganglioside type
?
hexadecanoyl-ceramide + H2O
hexadecanoate + sphingosine
i.e. C16-ceramide
-
-
r
L-erythro-sphingosine + fatty acid
?
-
low ceramide synthesis activity
-
-
?
myristic acid + sphingosine
N-myristoylsphingosine + H2O
-
highest ceramide synthesis activity with myristic acid
-
-
?
N-((2S,3R)-1,3-dihydroxy-5-((2-oxo-2H-chromen-7-yl)oxy)pentan-2-yl)palmitamide + H2O
palmitate + 2-amino-2,4-dideoxy-5-O-(2-oxo-2H-chromen-7-yl)-D-erythro-pentitol
-
-
-
-
?
N-arachidonoyl-dihydroceramide + H2O
dihydrosphingosine + arachidonate
-
-
-
-
?
N-arachidoyl-D-erythro-sphingosine + H2O
arachidonate + D-erythro-sphingosine
low activity
-
-
?
N-caproylsphingosine + H2O
caproate + sphingosine
-
about 25% activity compared to N-oleoylsphingosine
-
-
?
N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-dihydrosphingosine + H2O
12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoate + D-erythro-dihydrosphingosine
-
-
-
-
r
N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-phytosphingosine + H2O
12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanate + D-erythro-phytosphingosine
-
-
-
-
r
N-dodecanoyl-7-nitrobenz-2-oxa-1,3,4-diazole-D-erythro-sphingosine + H2O
12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanate + D-erythro-sphingosine
-
i.e. C12-NBD-Cer
-
-
r
N-hexanoyl-D-erythro-sphingosine + H2O
sphingosine + hexanoate
-
100.3% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine
-
-
?
N-lauroyl-D-erythro-sphingosine + H2O
sphingosine + dodecanoate
-
most efficient substrate, 293.2% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine
-
-
r
N-lignoceroyl-D-erythro-sphingosine + H2O
sphingosine + tetracosanoate
-
25.1% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine
-
-
?
N-myristoyl-D-erythro-sphingosine + H2O
myristate + D-erythro-sphingosine
-
best substrate
-
-
?
N-myristoyl-D-erythro-sphingosine + H2O
sphingosine + myristate
-
212.2% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine
-
-
?
N-nervonoyl-D-erythro-sphingosine + H2O
nervonoate + D-erythro-sphingosine
-
-
-
?
N-nervonoylsphingosine + H2O
nervonate + sphingosine
-
about 80% activity compared to N-oleoylsphingosine
-
-
?
N-oleoyl-D-erythro-sphingosine + H2O
oleate + D-erythro-sphingosine
-
-
-
?
N-palmitoyl sphingosine + H2O
palmitic acid + sphingosine
-
-
-
?
N-palmitoyl-D-erythro-sphingosine + H2O
D-erythro-sphingosine + palmitic acid
-
-
-
-
?
N-palmitoyl-D-erythro-sphingosine + H2O
sphingosine + palmitate
-
100% hydrolysis
-
-
r
N-palmitoylsphinganine + H2O
palmitate + sphinganine
low hydrolysis rate
-
-
?
N-stearoyl-D-erythro-sphingosine + H2O
sphingosine + stearate
-
98.5% hydrolysis compared to N-palmitoyl-D-erythro-sphingosine
-
-
?
N-stearoylsphinganine + H2O
stearate + sphinganine
low hydrolysis rate
-
-
?
N-stearoylsphingosine + H2O
stearate + sphingosine
less efficient hydrolysis than of N-lauroylsphingosine and N-palmitoylsphingosine
-
-
?
N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide + H2O
?
N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide + H2O
?
N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide + H2O
?
N-[(2S,3R,4E)-7-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-3-yl]oxy]-1,3-dihydroxyhept-4-en-2-yl]-11-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]undecanamide + H2O
?
N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]D-erythro-sphingosine + H2O
N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoate] + D-erythro-sphingosine
substrate C12-NBD ceramide
-
-
?
N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]lauroyl]-phytosphingosine + H2O
N-12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]laurate + phytosphingosine
-
i.e. D-ribo-C12-NBD-phytoceramide
-
-
?
N-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]lauroyl]-sphingosine + H2O
N-12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]laurate + sphingosine
i.e. C12-NBD-ceramide
-
-
r
octadecanoyl-ceramide + H2O
octadecanoate + sphingosine
i.e. C18-ceramide
-
-
r
palmitoyl-D-[erythro-9,10]sphingosine + H2O
palmitate + D-erythro-sphingosine
-
-
-
-
?
11-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-N-[(2S,3R,4E)-1,3-dihydroxy-7-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hept-4-en-2-yl]undecanamide + H2O
?
-
-
-
ir
11-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-N-[(2S,3R,4E)-1,3-dihydroxy-7-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hept-4-en-2-yl]undecanamide + H2O
?
-
-
-
ir
4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O
4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide
-
-
-
-
?
4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O
4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide
-
-
-
?
4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O
4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide
-
-
-
?
4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O
4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide
-
-
-
-
?
4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O
4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide
-
-
-
-
?
4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O
?
-
C12 substrate chain length
-
-
?
4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O
?
-
C12 substrate chain length
-
-
?
4-nitrobenzo-2-oxa-1,3-diazoyl-N-dodecanoylsphingosine + H2O
4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid + sphingosine
-
-
-
-
r
4-nitrobenzo-2-oxa-1,3-diazoyl-N-dodecanoylsphingosine + H2O
4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid + sphingosine
much faster hydrolysis than of N-lauroylsphingosine
-
-
?
C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O
?
substrate activity assay
-
-
?
C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O
?
substrate activity assay
-
-
?
C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O
?
substrate activity assay
-
-
?
C12-NBD-Cer + H2O
NBD-dodecanoic acid + sphingosine
-
-
-
?
C12:0-ceramide + H2O
laurate + sphingosine
-
preferred substrate
-
-
?
ceramide + H2O
?
ceramide in anionic liposomes. The negatively charged liposomes consisted of 25% mole bis(monoacylglycero)phosphate, 45% 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 20% cholesterol, and 10% 12:0 ceramide
-
-
?
ceramide + H2O
sphingosine + a fatty acid
-
-
sphongosine is an apoptosis mediator
-
?
ceramide + H2O
sphingosine + fatty acid
ceramidase activity on D-e-C6, D-e-C12, D-e-C16, D-e-C18, and D-e-C24:1-ceramide
-
-
?
ceramide + H2O
sphingosine + fatty acid
ceramidase activity on D-e-C6, D-e-C12, D-e-C16, D-e-C18, and D-e-C24:1-ceramide
-
-
?
ceramide + H2O
sphingosine + fatty acid
-
-
-
-
?
ceramide + H2O
sphingosine + fatty acid
-
for ceramide clearance, ceramidases hydrolyze the N-acyl fatty acid, yielding in the generation of sphingosine
-
-
?
ceramide + H2O
sphingosine + fatty acid
-
-
-
?
ceramide + H2O
sphingosine + fatty acid
-
the recombinant enzyme shows a broad subsrate specificity with ceramides, it shows good activity with ceramides containing C6-C24 fatty acids, and preference of ceramides with monounsaturated fatty acids than saturated fatty acids, overview
-
-
r
D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O
D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide
-
-
-
?
D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-C12-ceramide + H2O
D-erythro-4-nitrobenzo-2-oxa-1,3-diazole-dodecanoic acid + ceramide
-
-
-
?
D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O
D-erythro-sphingosine + ?
-
-
-
?
D-erythro-C12-4-nitrobenzo-2-oxa-1,3-diazole-ceramide + H2O
D-erythro-sphingosine + ?
-
substrate activity assay
-
-
?
D-erythro-C12-NBD-ceramide + H2O
D-erythro-sphingosine + 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid
maCER1 has a highly restricted substrate specificity, maCER1 has a major ceramidase activity and only a very minor reverse activity
-
-
r
D-erythro-C12-NBD-ceramide + H2O
D-erythro-sphingosine + 4-nitrobenzo-2-oxa-1,3-diazoyl-dodecanoic acid
-
substrate in activity assay
-
-
?
D-erythro-dodecanoyl-7-nitrobenz-2-oxa-1,3-diazol-4-yl-ceramide + H2O
12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoic acid + sphingosine
-
-
-
-
r
D-erythro-dodecanoyl-7-nitrobenz-2-oxa-1,3-diazol-4-yl-ceramide + H2O
12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoic acid + sphingosine
-
-
-
-
r
N-acyl-sphingosine + H2O
a carboxylate + sphingosine
-
the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the cellular levels of bioactive sphingolipid metabolites, ceramide, sphingosine, and sphingosine 1-phosphate, in the intestinal tract
-
-
?
N-acylsphingosine + H2O
a carboxylate + sphingosine
-
AC catalyzes the hydrolysis of ceramide to sphingosine and fatty acid
-
-
r
N-acylsphingosine + H2O
a carboxylate + sphingosine
-
CDase cleaves the N-acyl linkage of ceramide to produce sphingosine and free fatty acid
-
-
?
N-acylsphingosine + H2O
a carboxylate + sphingosine
CDase hydrolyzes the amide bond in ceramides to yield free fatty acid and sphingosine
-
-
?
N-acylsphingosine + H2O
a carboxylate + sphingosine
-
involved in the biosynthesis of ceramide, crucial enzyme for the regulation of the intracellular balance of the contents of ceramide, sphingosine and possibly sphingosine 1-phosphate
-
-
?
N-acylsphingosine + H2O
a carboxylate + sphingosine
-
involved in the biosynthesis of ceramide, crucial enzyme for the regulation of the intracellular balance of the contents of ceramide, sphingosine and possibly sphingosine 1-phosphate
-
-
r
N-acylsphingosine + H2O
a carboxylate + sphingosine
CDase catalyzes the hydrolysis of the N-acyl linkage of ceramide to produce sphingosine
-
-
?
N-acylsphingosine + H2O
a carboxylate + sphingosine
-
CDase cleaves the N-acyl linkage of ceramide into sphingosine and free fatty acid, brain CDase is able to catalyze the reverse reaction, i.e. ceramide synthesis
-
-
r
N-acylsphingosine + H2O
a carboxylate + sphingosine
-
CDase cleaves the N-acyl linkage of ceramide to produce sphingosine and free fatty acid
-
-
?
N-acylsphingosine + H2O
carboxylate + sphingosine
the carboxylate is a fatty acid
-
-
?
N-acylsphingosine + H2O
carboxylate + sphingosine
-
the carboxylate is a fatty acid
-
-
?
N-acylsphingosine + H2O
carboxylate + sphingosine
the carboxylate is a fatty acid
-
-
?
N-acylsphingosine + H2O
carboxylate + sphingosine
the carboxylate is a fatty acid
-
-
?
N-acylsphingosine + H2O
carboxylate + sphingosine
the carboxylate is a fatty acid
-
-
r
N-acylsphingosine + H2O
carboxylate + sphingosine
the carboxylate is a fatty acid, Ypc1p can catalyse the reverse reaction, i.e. the condensation of non-esterified fatty acids with phytosphingosine or dihydrosphingosine. The cysteine residues at positions 27 and 219 are required for reverse ceramidase activity
-
-
r
N-acylsphingosine + H2O
carboxylate + sphingosine
the carboxylate is a fatty acid. The cysteine residues at positions 27 and 219 are required for reverse ceramidase activity
-
-
r
N-hexanoyl-D-erythro-sphingosine + H2O
hexanoate + D-erythro-sphingosine
-
-
-
-
?
N-hexanoyl-D-erythro-sphingosine + H2O
hexanoate + D-erythro-sphingosine
-
-
-
?
N-lauroyl-D-erythro-sphingosine + H2O
laurate + D-erythro-sphingosine
-
the enzyme catalyzes also the reverse reaction of ceramide synthesis
-
-
r
N-lauroyl-D-erythro-sphingosine + H2O
laurate + D-erythro-sphingosine
best substrate
-
-
?
N-lauroylsphingosine + H2O
laurate + sphingosine
more efficient hydrolysis than of N-palmitoylsphingosine and N-stearoylsphingosine, less efficient hydrolysis than of 4-nitrobenzo-2-oxa-1,3-diazoyl-N-dodecanoylsphingosine
-
-
?
N-lauroylsphingosine + H2O
laurate + sphingosine
-
about 95% activity compared to N-oleoylsphingosine
-
-
?
N-lauroylsphingosine + H2O
lauric acid + sphingosine
-
optimal substrate, AC also efficiently catalyzes ceramide synthesis
-
-
r
N-lignoceroyl-D-erythro-sphingosine + H2O
lignocerate + D-erythro-sphingosine
-
low activity
-
-
?
N-lignoceroyl-D-erythro-sphingosine + H2O
lignocerate + D-erythro-sphingosine
low activity
-
-
?
N-oleoyl-D-erythro-ceramide + H2O
oleate + D-erythro-sphingosine
-
common substrate in erythrocytes
-
-
?
N-oleoyl-D-erythro-ceramide + H2O
oleate + D-erythro-sphingosine
-
i.e. D-e-C18:1-ceramide, common substrate in erythrocytes
-
-
?
N-oleoyl-D-erythro-ceramide + H2O
oleate + D-erythro-sphingosine
-
common substrate in erythrocytes
-
-
?
N-oleoyl-D-erythro-ceramide + H2O
oleate + D-erythro-sphingosine
-
i.e. D-e-C18:1-ceramide, common substrate in erythrocytes
-
-
?
N-palmitoyl-D-erythro-sphingosine + H2O
palmitate + D-erythro-sphingosine
-
-
-
-
?
N-palmitoyl-D-erythro-sphingosine + H2O
palmitate + D-erythro-sphingosine
-
-
-
?
N-palmitoylsphingosine + H2O
palmitate + sphingosine
less efficient hydrolysis than of N-lauroylsphingosine, more efficient hydrolysis than of N-stearoylsphingosine
-
-
?
N-palmitoylsphingosine + H2O
palmitate + sphingosine
-
about 65% activity compared to N-oleoylsphingosine
-
-
?
N-stearoyl-D-erythro-sphingosine + H2O
stearate + D-erythro-sphingosine
-
-
-
-
?
N-stearoyl-D-erythro-sphingosine + H2O
stearate + D-erythro-sphingosine
low activity
-
-
?
N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide + H2O
?
5.6% hydrolysis
-
-
ir
N-[(2S,3R,4E)-1,3-dihydroxy-14-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]tetradec-4-en-2-yl]hexadecanamide + H2O
?
6.2% hydrolysis
-
-
ir
N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide + H2O
?
6.1% hydrolysis
-
-
ir
N-[(2S,3R,4E)-1,3-dihydroxynonadec-4-en-2-yl]-12-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]dodecanamide + H2O
?
63.3% hydrolysis
-
-
ir
N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide + H2O
?
3.9% hydrolysis
-
-
ir
N-[(2S,3R,4E)-13-[[9-(ethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-1,3-dihydroxytridec-4-en-2-yl]hexadecanamide + H2O
?
6.3% hydrolysis
-
-
ir
N-[(2S,3R,4E)-7-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-3-yl]oxy]-1,3-dihydroxyhept-4-en-2-yl]-11-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]undecanamide + H2O
?
fluorescently labelled ceramide analogue, 4.0% hydrolýsis
-
-
ir
N-[(2S,3R,4E)-7-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-3-yl]oxy]-1,3-dihydroxyhept-4-en-2-yl]-11-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]undecanamide + H2O
?
fluorescently labelled ceramide analogue, 5.8% hydrolýsis
-
-
ir
palmitoyl sphingosine + H2O
palmitate + sphingosine
substrate activity assay
-
-
?
palmitoyl sphingosine + H2O
palmitate + sphingosine
-
substrate activity assay
-
-
?
additional information
?
-
-
ceramidase catalyzes the hydrolysis of ceramide to form sphingosine
-
-
?
additional information
?
-
ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate
-
-
?
additional information
?
-
-
ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate
-
-
?
additional information
?
-
ceramidases are a group of enzymes that catalyze the hydrolysis of ceramides to generate sphingosine, which is phosphorylated to form sphingosine 1-phosphate
-
-
?
additional information
?
-
-
critical role of AC in the regulation of sphingolipid metabolism
-
-
?
additional information
?
-
acidic and alkaline isozyme activities increase with keratinocyte differentiation, while the activities of phytoalkaline and neutral isozymes decrease, overview
-
-
?
additional information
?
-
CER2 catalyzes the hydrolysis of ceramides derived from both the de novo and sphingomyelin breakdown pathways, Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and sphingosine 1-phosphate
-
-
?
additional information
?
-
-
CER2 catalyzes the hydrolysis of ceramides derived from both the de novo and sphingomyelin breakdown pathways, Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and sphingosine 1-phosphate
-
-
?
additional information
?
-
-
cooperative prosurvival activity by MAP kinases ERK and Akt in human alveolar macrophages is dependent on high levels of acid ceramidase activity, overview, blocking of acid ceramidase but not sphingosine kinase activity in alveolar macrophages leads to decreased ERK and Akt activity and induction of cell death, sphingosine and L-threo-dihydrosphingosine reverse the antisurvival effects of acid ceramidase inhibition in alveolar macrophages
-
-
?
additional information
?
-
-
diacylglycerol assay, i.e. ceramide:DAG assay
-
-
?
additional information
?
-
-
acid ceramidase plays a critical role in the regulation of ceramide and sphingosine metabolism
-
-
?
additional information
?
-
-
ASAH1 hydrolyzes ceramides and regulates neuronal development, and its deficiency often results in mental retardation. Results of linkage disequilibrium and polymorphism analyses suggest that a particular group of haplotypes in ASAH1 represents a signature of recent positive Darwinian selection
-
-
?
additional information
?
-
-
neutral ceramidase plays a major role in ceramide metabolism in the human gut
-
-
?
additional information
?
-
-
acid ceramidase deacylates ceramide and yields sphingosine
-
-
?
additional information
?
-
-
ceramidase catalyzes the hydrolysis of ceramide into sphingosine and a free fatty acid
-
-
?
additional information
?
-
-
ceramidase catalyzes the hydrolysis of ceramide to form sphingosine
-
-
?
additional information
?
-
-
ACER3 can catalyze the hydrolysis of unsaturated long-chain dihydroceramides as efficiently as unsaturated long-chain ceramides. ACER1 and ACER2, the homologues of ACER3, also catalyzed the hydrolysis of certain dihydroceramide species to generate dihydrosphingosine
-
-
?
additional information
?
-
-
ACER2 shows a broader substrate specificity catalyzing the hydrolysis of various ceramides. The luminal ACER2 N-terminal tail, comprising 36 amino acid residues, is important for ACER2 activity, but the terminal 13 amino acid residues are not required for its activity
-
-
?
additional information
?
-
-
ACER3 hydrolyzes C18:1-, C20:1-, C20:4-ceramides, C18:1-, C20:1-, C20:4-dihydroceramides, and C18:1-, C20:1-, C20:4-phytoceramides in vitro
-
-
?
additional information
?
-
-
only the natural D-erythro-ceramide isomer is used as substrate of the four stereoisomers of ceramide. Dihydroceramide or phytoceramide forms, shortening the length of the alkyl backbone, methylation of the primary or secondary hydroxyl groups results in reduction or loss of the neutral ceramidase activity
-
-
?
additional information
?
-
only the natural D-erythro-ceramide isomer is used as substrate of the four stereoisomers of ceramide. Dihydroceramide or phytoceramide forms, shortening the length of the alkyl backbone, methylation of the primary or secondary hydroxyl groups results in reduction or loss of the neutral ceramidase activity
-
-
?
additional information
?
-
only the natural D-erythro-ceramide isomer is used as substrate of the four stereoisomers of ceramide. Dihydroceramide or phytoceramide forms, shortening the length of the alkyl backbone, methylation of the primary or secondary hydroxyl groups results in reduction or loss of the neutral ceramidase activity
-
-
?
additional information
?
-
only the natural D-erythro-ceramide isomer is used as substrate of the four stereoisomers of ceramide. Dihydroceramide or phytoceramide forms, shortening the length of the alkyl backbone, methylation of the primary or secondary hydroxyl groups results in reduction or loss of the neutral ceramidase activity
-
-
?
additional information
?
-
-
short acyl-chain ceramides (i.e. C16 acyl chain) are poor substrates for acid ceramidase
-
-
?
additional information
?
-
short acyl-chain ceramides (i.e. C16 acyl chain) are poor substrates for acid ceramidase
-
-
?
additional information
?
-
short acyl-chain ceramides (i.e. C16 acyl chain) are poor substrates for acid ceramidase
-
-
?
additional information
?
-
short acyl-chain ceramides (i.e. C16 acyl chain) are poor substrates for acid ceramidase
-
-
?
additional information
?
-
acid ceramidase catalyzes the hydrolysis of ceramide to constituent sphingoid base, sphingosine, and fatty acid
-
-
?
additional information
?
-
the N-acyl moiety of ceramide is cleaved by ceramidases to form the lysolipid sphingosine that undergoes further phosphorylation to sphingosine-1-phosphate
-
-
?
additional information
?
-
design and synthesis of a set of fluorescently labeled ceramides as substrates for acid and neutral ceramidases, overview. Although both the dyes, (acyl-(4-nitro-2,1,3-benzoxadiazol-7-yl)aminoethyl)-ceramide and acyl-Nile red-ceramide do not have any noticeable preference for the substitution at acyl or sphingosine part in ceramide towards hydrolysis by acid ceramidase, the ceramides with acyl-substituted NBD and Sph-substituted NR dyes have been found to be a better substrate for neutral ceramidase
-
-
?
additional information
?
-
design and synthesis of a set of fluorescently labeled ceramides as substrates for acid and neutral ceramidases, overview. Although both the dyes, (acyl-(4-nitro-2,1,3-benzoxadiazol-7-yl)aminoethyl)-ceramide and acyl-Nile red-ceramide do not have any noticeable preference for the substitution at acyl or sphingosine part in ceramide towards hydrolysis by acid ceramidase, the ceramides with acyl-substituted NBD and Sph-substituted NR dyes have been found to be a better substrate for neutral ceramidase
-
-
?
additional information
?
-
the enzyme directly interacts with and binds steroidogenic factor 1, interaction site analysis, overview
-
-
?
additional information
?
-
-
the enzyme directly interacts with and binds steroidogenic factor 1, interaction site analysis, overview
-
-
?
additional information
?
-
ADIPOR2 may have a preference for C18 ceramide substrate, but can also hydrolyse shorter (C6 ceramide) and longer (C24 ceramide) substrates, but to a lesser extent. Low overall ceramidase catalytic activity of ADIPOR2. Substrate binding structure, overview. The substrate amide carbonyl contacts the R278TM5 and Y328TM6 side chains, which are typical carbonyl-polarizing and oxyanion-stabilizing residues in zinc-dependent hydrolases
-
-
?
additional information
?
-
the enzyme performs autocatalysis. Substrate modeling suggests distinct catalytic mechanisms for substrate hydrolysis versus autocleavage
-
-
?
additional information
?
-
the enzyme has broad substrate specificity with preference for ceramides with a medium acyl-chain or a mono unsaturated long acyl-chain. The enzyme prefers ceramides with unsaturated acyl-chains over ceramides with saturated acyl-chains of the same length, and it does not use phytoceramides as substrates, suggesting that LsnCer is a ceramide-specific ceramidase
-
-
?
additional information
?
-
-
the enzyme has broad substrate specificity with preference for ceramides with a medium acyl-chain or a mono unsaturated long acyl-chain. The enzyme prefers ceramides with unsaturated acyl-chains over ceramides with saturated acyl-chains of the same length, and it does not use phytoceramides as substrates, suggesting that LsnCer is a ceramide-specific ceramidase
-
-
?
additional information
?
-
maCER1 may play a role in regulating the levels of bioactive lipids ceramide and sphingosine-1-phosphate, as well as complex sphingolipids, maCER1 may have a specific role in skin function
-
-
?
additional information
?
-
-
maCER1 may play a role in regulating the levels of bioactive lipids ceramide and sphingosine-1-phosphate, as well as complex sphingolipids, maCER1 may have a specific role in skin function
-
-
?
additional information
?
-
not: D-erythro-C12-NBD-dihydroceramide, D-ribo-C12-NBD-phytoceramide, L-threo-C12-NBD-ceramide
-
-
?
additional information
?
-
-
not: D-erythro-C12-NBD-dihydroceramide, D-ribo-C12-NBD-phytoceramide, L-threo-C12-NBD-ceramide
-
-
?
additional information
?
-
-
acid ceramidase, but not acid sphingomyelinase, is required for tumor necrosis factor-alpha-induced prostaglandin PGE2 production, overview
-
-
?
additional information
?
-
-
the enzyme is involved in ceramide metabolism at the plasma membrane and in extracellular milieu, hydrolysis of ceramide on the cell surface, overview, involvement of the enzyme in stimulus-induced Cer metabolism by doxorubicin
-
-
?
additional information
?
-
-
the enzyme is involved in degradation of epidermal ceramides, ceramidase activity is not age-dependent
-
-
?
additional information
?
-
-
ceramidase catalyzes the hydrolysis of ceramide to form sphingosine and free fatty acid
-
-
?
additional information
?
-
-
cleaves the N-acyl linkage of ceramides into a sphingosine base and a free fatty acid
-
-
?
additional information
?
-
the N-acyl moiety of ceramide is cleaved by ceramidases to form the lysolipid sphingosine that undergoes further phosphorylation to sphingosine-1-phosphate
-
-
?
additional information
?
-
-
recombinant NlnCDase shows a broad capacity to hydrolyze different ceramide varieties and has a preference for the short and medium chain ceramide, the substrate preference in descending order is C12-ceramide, C6-ceramide, and C16-ceramide. The enzyme shows low activity for the very-short chain C2-ceramide and the long chain substrates C20-ceramide and C24-ceramide
-
-
?
additional information
?
-
CDase may be a virulence factor
-
-
?
additional information
?
-
-
the C-terminal tail is essential for enzyme activity and correct folding
-
-
?
additional information
?
-
-
ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa
-
-
?
additional information
?
-
-
ceramidase enhances cytotoxicity induced by hemolytic phospholipase C
-
-
?
additional information
?
-
ceramidase catalyzes the hydrolysis of ceramide to generate sphingosine and fatty acid, also found to catalyze the reverse reaction
-
-
?
additional information
?
-
-
ceramidase catalyzes the hydrolysis of ceramide to generate sphingosine and fatty acid, also found to catalyze the reverse reaction
-
-
?
additional information
?
-
-
the Pseudomonas neutral CDase can hydrolyze ceramide in intact erythrocytes, leading to hemolysis. The reaction mechanism for ceramide hydrolysis in vivo appears to be the same as that for ceramide hydrolysis in vitro because the mutation of residues surrounding the catabolic zinc ion abolish the hemolytic activity along with ceramide hydrolysis by CDase in intact erythrocytes
-
-
?
additional information
?
-
-
the enzyme can hydrolyze human skin-specific omega-hydroxyacyl ceramides
-
-
?
additional information
?
-
-
the enzyme is able to effectively degrade ceramide in the presence of Staphylococcus aureus-derived lipids or neutral detergents
-
-
?
additional information
?
-
-
ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa
-
-
?
additional information
?
-
-
ceramidase enhances cytotoxicity induced by hemolytic phospholipase C
-
-
?
additional information
?
-
-
neutral ceramidase is involved in the regulation of ceramide-mediated signaling
-
-
?
additional information
?
-
-
no substrate in the ceramide synthesis reaction: palmitoyl-CoA
-
-
?
additional information
?
-
not: glycosphingolipids, e.g. GalCer, sulfatide, Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide, sphingomyelin
-
-
?
additional information
?
-
-
the C-terminal residues Ile-758 and Phe-756 are essential for enzyme function, the C-terminal tail is indispensable for the correct folding, localization and enzyme activity
-
-
?
additional information
?
-
-
the enzyme is important in digestion of sphingolipids
-
-
?
additional information
?
-
-
neutral ceramidase is a key enzyme for hydrolysis of sphingomyelin in the gut
-
-
?
additional information
?
-
ceramidase catalyzes the hydrolysis of ceramide to generate sphingosine and fatty acid, also found to catalyze the reverse reaction
-
-
?
additional information
?
-
ceramidase deacylates ceramide to form sphingosine
-
-
?
additional information
?
-
-
long chain dihydroceramide dihydro-hexanoyl-D-erythro-sphingosine is resistant to the enzyme
-
-
?
additional information
?
-
-
the enzyme prefers medium-chain or long chain ceramides over short-chain ceramides as substrates
-
-
?
