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Literature summary for 3.5.1.23 extracted from
- Purification and characterization of human intestinal neutral ceramidase (2007), Biochimie, 89, 950-960.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CHAPS | 20 mM, 50% inhibition | Homo sapiens |  |
| cholesterol | 0.08 mM or below, decreases the hydrolysis of ceramide by a maximum of two-thirds | Homo sapiens | |
| Cu2+ | - |
Homo sapiens | |
| glycodeoxycholate | - |
Homo sapiens | |
| Mg2+ | 5 mM | Homo sapiens | |
| Mn2+ | 5 mM | Homo sapiens | |
| taurocholate | 20 mM, 50% inhibition | Homo sapiens | |
| taurodeoxycholate | - |
Homo sapiens | |
| Zn2+ | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01302 | - |
N-octanoylsphingosine | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 116000 | - |
x * 116000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | neutral ceramidase plays a major role in ceramide metabolism in the human gut | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | deglycosylation does not affect the activity | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| ileostomal fluid | - |
Homo sapiens | - |
| intestine | neutral ceramidase is expressed in the intestine and released in the intestinal lumen | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.647 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | neutral ceramidase plays a major role in ceramide metabolism in the human gut | Homo sapiens | ? | - |
? | |
| N-octanoylsphingosine + H2O | - |
Homo sapiens | octanoic acid + sphingosine | - |
r | |
| N-oleoylsphingosine + H2O | - |
Homo sapiens | oleic acid + sphingosine | - |
r | |
| N-palmitoylsphingosine + H2O | - |
Homo sapiens | palmitic acid + sphingosine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 116000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| neutral ceramidase | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
synthesis of N-palmitoylsphingosine | Homo sapiens |
| 7.5 | - |
hydrolysis of N-octanoylsphingosine | Homo sapiens |
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Release 2023.1 (January 2023)
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